黄曲霉核糖体蛋白基因在不同生长时期的表达分析

Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus

核糖体是生物合成蛋白质的主要场所。核糖体蛋白是核糖体的重要组分之一。为了探究黄曲霉核糖体蛋白与其生长发育之间的关系,分别收集处于菌丝期、孢子期、菌核期的黄曲霉。在提取了总RNA和反转录获得了c DNA后,利用荧光定量RT-PCR对74个黄曲霉核糖体蛋白基因的表达量进行分析。结果表明,在所有被检测的核糖体蛋白基因中,没有一个基因的表达量在3个时期都是恒定的。与菌丝期相比,在孢子期,有54个核糖体蛋白基因的表达量发生上调,而表达量下调的基因只有2个;在菌核期,有57个基因的表达量上调,而有12个基因的表达量下调。GO功能分析表明,核糖体蛋白基因富集在结构分子活性,结合,细胞,大分子复合物,细胞器,细胞部分,细胞器部分,细胞过程,代谢过程。可见,核糖体蛋白与黄曲霉的生长发育存在紧密联系。

Ribosome is a major organelle of protein synthesis in organism. Ribosomal protein is one of main components of ribosome.In order to explore the relationship between Aspergillus flavus ribosomal protein and its development,A. flavus were collected at mycelialstage,spore stage,and sclerotial stage. After total RNA extraction and the c DNA preparation,the expression levels of 74 genes of A. flavusribosomal protein were detected by quantitative RT-PCR. The results showed that,none of the tested genes was expressed invariably at all threestages. Compared with mycelial stage,the expression levels of 54 ribosomal protein genes were up-regulated at the spore stage,while 2 geneswere down-regulated. At sclerotial stage,the expression levels of 57 genes were up-regulated,while 12 genes were down-regulated. The GOfunctional analysis showed that the ribosomal protein genes were enriched into structural molecular activity,binding,cell,macromolecularcomplex,organelle,cell part,organelle part,cell process,and metabolic process. It can be concluded that ribosomal proteins are closelyrelated to the growth and development of A. flavus.