大鼠血浆中沃诺拉赞的溶剂诱导相变萃取-HPLC测定方法的建立

Determination of vonoprazan in rat plasma by solvent induced phase transition extraction-HPLC method

目的:采用溶剂诱导相变萃取-HPLC技术建立测定大鼠血浆中的沃诺拉赞浓度的方法。方法:色谱柱为Syncronis C_(18)色谱柱(250mm×4.6mm,5μm),柱温为40℃,流动相为20 mM NaH_2PO_4溶液(含0.1%甲酸)-乙腈(95:5,v/v),流速为1 mL·min^(-1),检测波长为230 nm。血浆样品采用溶剂诱导相变萃取法。结果:建立的方法专属性、线性、精密度与准确度、稳定性符合均生物样品测定要求。结论:溶剂诱导相变萃取-HPLC测定方法适用于大鼠血浆中沃诺拉赞浓度的测定。

Objective： To establish a solvent induced phase transition extraction - HPLC method for the determination of vonoprazan in rat plasma. Method： The chromatographic conditions were as follows： a Syncronis C18 chromatographic column （250mm×4.6mm, 5μm） was used at 40℃. The mobile phase consisted of 20 mM potassium dihydrogen phosphate buffer solution （containing 0.1% formic aeid）-acetonitrile （95：5, v/v ） with the flow rate at 1 mL· min^-1. The detection wavelength was 230 nm. The plasma samples were extracted by solvent induced phase transition extraction method. Result： The selectivity, linearity, precision, accuracy and stability of the established method were completely within the acceptance limits for biological samples. Conclusion： The solvent induced phase transition extraction - HPLC method was suitable for determination of vonoprazan in rat plasma.