A-40926混合物中B_0组分的分离纯化

Separation and purification of component B_0 in antibiotic A-40926

目的开发一种高纯度A-40926混合物中B_0组分(以下简称A-40926 B_0)的分离纯化工艺。方法采用中压色谱系统,以聚合物微球为载体,A-40926 B_0的收率为指标,筛选聚合物微球的类型,优化洗脱条件。结果型号为Uni PS30-300的微球为最佳层析介质,上样量为10mg(每毫升填料),洗脱剂依次为体积分数30%,45%的乙醇溶液,流速为1.5BV/h(BV:层析柱体积);所得产品纯度大于95%,层析总收率大于75%。结论此种高纯度A-40926 B_0的分离纯化方法简单易行,收率稳定,适于工业化生产。

Objective To develop the separation and purification process of B0 component in the A-40926 mixture （here in after referred to as A-40926 B0）. Methods Using medium pressure chromatography system, the polymer microspheres were used as carriers and the yield of A-40926 B0 as indexes. The types of polymer microspheres were screened and the elution conditions were optimized. Results The type of UniPS30-300 polymer particles were selected as the best chromatographic media. The optimal conditions were as follows： The sample loading amount was 10mg （per lmL filler）, and 30% and 45% ethanol solution was used as the elution solution at a flow rate of 1.SBV/h （BV： column volume）. The purity of the product was higher than 95% and the yield was above 75%. Conclusion The established purification process ofA-40926 B0 was simple to operate and the yield was high and feasible. Thus the orocess was suitable for industrialized production.