Flotillin-2在胃癌组织中的表达及其临床意义

Expression and clinical significance of Flotillin-2 in gastric cancer tissues

目的:检测脂筏标记蛋白-2(Flotillin-2,Flot-2)在胃癌组织中的表达水平,分析Flot-2的表达与胃癌临床病理特征及预后的关系。方法:选取南昌大学第二附属医院胃肠外科于2009年1月至2010年4月行手术切除的胃癌组织112例及与其配对的癌旁组织,采用免疫组织化学法检测肿瘤组织中Flot-2的表达水平,采用Spearman检验Flot-2表达与胃癌临床病理特征及预后的关系,采用Kaplan-Meier法及Log-Rank检验分析其生存数据。结果:Flot-2阳性表达呈黄色颗粒,主要在细胞质中表达,胃癌组织中的表达量明显高于癌旁组织(53.57%vs 46.43%,P<0.05)。Flot-2表达与患者的性别、年龄、肿瘤位置、分化程度无显著关联(P>0.05),与肿瘤大小、浸润深度、淋巴结转移、远处转移及AJCC分期均显著关联(均P<0.01)。Flot-2低表达组患者的5年总体生存率明显高于高表达组(P<0.01)。Cox回归分析显示,远处转移、AJCC分期和Flot-2蛋白表达水平为胃癌患者预后的独立危险因素。结论:胃癌组织中高表达Flot-2,其与患者不良预后密切相关,是胃癌预后的独立危险因素,有望成为胃癌治疗的潜在新靶点。

Objective： To investigate the expression of Flotillin-2（Flot-2） protein in gastric cancer tissues and its relationship with clinicopathological features and prognosis of gastric cancer（GC） patients. Methods： 112 samples of gastric cancer tissue and the corresponding paracancerous tissue that resected at the gastrointestinal surgery department of the Second Affiliated Hospital of Nanchang University between January 2009 and April 2010 were collected for this study. The expression of Flot-2 protein in tumor tissues was detected by immunohistochemistry. The survival data were analyzed by Kaplan-Meier and Log-Rank test, and the survival curve was plotted. Spearman correlation analysis was used to examine the relationship between Flot-2 protein expression and clinicopathological characteristics and prognosis of GC patients. Results： In gastric cancer tissues, Flot-2 was primary stained in cytoplasm. Level of Flot-2 was significantly higher in gastric cancer tissues compared with that in paracancerous tissues（53.57% vs 46.43%, P〈0.05）. Expression of Flot-2 in tumor tissues was significantly associated with tumor size, depth of invasion, lymph node metastasis, distant metastasis and AJCC stage（all P〈0.01）, but not with gender, age, differentiation degree and tumor location（P〉0.05）. Moreover, survival analysis showed that the overall survival of patients with low Flot-2 expression was significantly higher than that of the patients with high level（P〈0.01）. Cox regression analysis indicated that distant metastasis, AJCC stage and Flot-2 expression were the independent risk factors for the prognosis of GC patients. Conclusion： Flot-2 protein was highly expressed in gastric cancer tissues and closely correlated with the poor prognosis of GC patients; Flot-2 is an independent risk factor for GC prognosis and may be served as a potential therapeutic target for gastric cancer.