TLR9基因rs187084单核苷酸多态性与中国藏族人群结核易感性研究

Association of the TLR9 gene rs187084 single nucleotide polymorphism with tuberculosis in the Chinese Tibetan population

目的目前关于藏族人群Toll样受体9(TLR9)SNP与结核病相关性的研究较少。文中旨在探讨中国藏族人群Toll样受体9(TLR9)基因多态性与结核病易感性的相关性。方法选择2013年2月至2015年8月于四川省阿坝藏族羌族自治州人民医院就诊的中国藏族人群1216例。其中613例结核病患者为结核病组,603例体检健康者为对照组。应用多重连接酶检测反应(MLDR)技术,检测TLR9(rs187084、rs5743836)多态性位点的基因型,并采用Logistic回归分析多态性位点与藏族人群结核病的易感性。结果 SNP位点rs187084 G/A的等位基因频率分布在结核病组、对照组间差异无统计学意义(P=0.668)。rs187084的基因型与藏族人群结核病的遗传易感无相关性(P>0.05)。rs187084位点在显性模型、隐性模型及加性模型分析中差异均无统计学意义(P>0.05)。结论 TLR9基因rs187084多态位点与中国藏族人群的结核病遗传易感性无关联。

Objective The purpose of this study was to investigate the correlation between single nucleotide polymorphisms（ SNP） of Toll-like receptor 9（ TLR9） and tuberculosis in the Chinese Tibetan population. Methods A total of 613 active tuberculosis patients and 603 healthy controls were enrolled in this case-control study. Two SNPs in TLR9（ rs187084 and rs5743836） were genotyped using the multiplex ligation detection reaction technique. The association of the SNPs with the susceptibility of TB was inves-tigated using logistic regression analysis. Results There was no statistically significant difference in the allele distribution of rs187084 G/A between the TB patients and the healthy controls（ P = 0.668） or correlation between the genotype of rs187084 and the genetic susceptibility of TB（ P〈0. 05）. Dominant,recessive and additive models showed no association of this SNP with TB,either（ P〉 0. 05）.Conclusion The rs187084 polymorphism of TLR9 is not correlated with the genetic susceptibility of tuberculosis in the Chinese Tibetan population.