儿童喉乳头状瘤组织中Toll样受体4及激活蛋白-1表达及相关性研究

Correlation of the expression of Toll-like receptor 4 and activator protein-1 in juvenile laryngeal papilloma

目的:研究儿童喉乳头状瘤(juvenile onset laryngeal papilloma,JLP)中Toll样受体4(Toll-like receptor 4,TLR4)、激活蛋白-1(activator protein-1,AP-1)(通常是c-Jun和c-Fos的异源二聚体)蛋白的表达及其相互关系。方法:应用免疫组化法及RT-PCR检测JLP组织中TLR4和AP-1(c-Jun、c-Fos)的表达情况,分析两者表达的相关性及与JLP复发的相关性。结果:儿童JLP组织中TLR4和AP-1(c-Jun、cFos)蛋白和mRNA均为阳性表达,与无复发组比较,复发组有显著性增高(P<0.01),与正常对照组比较,JLP两组均有显著性增高(P<0.01)。其中TLR4蛋白复发组和无复发组阳性率分别为83.33%和58.33%,cJun、c-Fos蛋白复发组阳性率分别为86.67%和83.33%,无复发组阳性率均为66.67%。TLR4和AP-1(c-Jun、c-Fos)蛋白表达均与JLP的复发有明显相关性(P<0.05或者P<0.01);JLP组织中TLR4和AP-1(c-Jun、c-Fos)表达两者呈正相关(P<0.01)。结论:TLR4及AP-1的高表达可能在JLP的发生发展机制中起着重要作用。

Objective：To study the expression and correlation of Toll-like receptor 4（ TLR4）,activator protein-1（ AP-1）（ usually for c-Jun and c-Fos heterologous dimers） protein in juvenile onset laryngeal papilloma（ JLP）.Methods：Adopt the immunohistochemical method and RT-PCR to detect the expression of TLR4 and AP-1（ c-Jun,c-Fos） in JLP tissue,analyse the correlation between the expression of two of them and the correlation with JLP relapse.Results：TLR4 and AP-1（ c-Jun,c-Fos） protein and mRNA in JLP tissue were all expressed as positive.Compared with no relapse group,the relapse group was significantly increased（ P〈0.01）,compared with normal control group,two JLP groups all were significantly increased（ P〈0.01）.The positive rates of TLR4 protein in relapse group and no relapse group were 83.33% and 58.33% respectively.The positive rates of c-Jun and c-Fos protein in relapse group were 86.67% and 83.33% respectively,and the positive rates in no relapse group were66.67%.Protein expressions of TLR4 and AP-1（ c-Jun,c-Fos） all had obvious correlation with the relapse of JLP（ P〈0.05 or P〈0.01）.TLR4 and AP-1（ c-Jun,c-Fos） in JLP tissue showed that they were positively correlated（ P〈0.01）.Conclusion：High expression of TLR4 and AP-1 may play an important role in the occurance and development mechanism of JLP.