不育男性精子DNA碎片指数与年龄和精液参数相关性分析

Correlation of sperm DNA fragmentation index with age and semen parameters in infertile men

目的:通过对男性不育患者进行精子染色质结构分析(SCSA),探讨精子DNA碎片指数(DFI)与不育患者年龄、精子浓度及精子活率之间的关系。方法:531份精液样本来自2016年1月至2017年6月在武汉同济生殖医学专科医院就诊的男性不育患者。用计算机辅助精液分析系统(CASA)对精子浓度、活率和精液体积等参数进行常规分析,同时利用流式细胞术检测精子DFI,并分析精子DFI与受检者的年龄、精子浓度及精子活率的相关性。结果:随着患者年龄的增加,患者中精子DFI≤15%的比例明显减少,精子DFI≥25%的比例明显增加,21~30岁、31~40岁、41~52岁年龄组患者精子DFI≤15%分别为83.8%、47.8%、20.3%,精子DFI≥25%分别为8.8%、14.5%、72.9%,相关性分析表明患者年龄与精子DFI成正相关关系(r=0.653,P<0.01)。而随着患者精子浓度和精子活率的增加,患者中精子DFI≤15%的比例明显增加,15%<精子DFI<25%和精子DFI≥25%的比例明显减少,相关性分析表明精子浓度、精子活率分别与精子DFI成负相关(r=-0.246,P<0.01;r=-0.406,P<0.01)。结论:精液中精子DFI与患者年龄、精子浓度及精子活率均显著相关,精子DFI可以作为评估精液质量的一个重要指标。综合分析精子DFI及精液常规参数能够对男性生育力进行更全面的评估。

Objective： To explore the correlation of the sperm DNA fragmentation index（ DFI） with age,sperm concentration and sperm motility in infertile men. Methods： We collected semen samples from 531 infertile males in our hospital from January 2016 to June 2017. We determined the semen parameters using the computer-assisted semen analysis system,measured the sperm DFI by sperm chromatin structure assay,and analyzed the correlation of the sperm DFI with the age,sperm concentration and sperm motility of the patients. Results： With the increase of age,the infertile males showed a significantly decreased proportion of the sperm with a DFI ≤15% and elevated proportion of the sperm with a DFI ≥25%,with a positive correlation between age and sperm DFI（ r =0. 653,P〈0. 01）. With the increase of sperm concentration and motility,however,the proportion of the sperm with a DFI ≤15%was remarkably increased while that of the sperm with 15% DFI 25% and ≥25% markedly reduced,both sperm concentration and motility negatively correlated with the sperm DFI（ r =-0. 246,P〈0. 01 and r =-0. 406,P〈0. 01）. Conclusion： The sperm DFI is significantly correlated with age,sperm concentration and sperm motility,and therefore can be used as an important index for the evaluation of semen quality. A comprehensive analysis of the sperm DFI and semen parameters may contribute to an accurate assessment of male fertility.