具有止血作用的纤维蛋白凝胶的制备及表征

The Preparation and Characterization of Fibrin-gel for Hemostatic Application

主要研究一种具有止血作用的生物材料。本文以纤维蛋白原和凝血酶为组分,制成纤维蛋白凝胶,通过材料学表征、止血评价及动物实验研究该凝胶材料的止血效果。将纤维蛋白原溶液和凝血酶溶液按一定比例混合,制备出体积比为1∶1、1∶3、1∶5的3种不同纤维蛋白凝胶。通过材料学表征发现,凝胶内部为多孔纤维状网络结构,其中1∶3的凝胶内部孔径最小,溶胀度最大。止血评价实验发现,凝胶能显著激活血小板,表面极易形成血栓。乳鼠创伤实验发现,为1∶5的凝胶对伤口闭合有一定促进作用。综上,本文所制备凝胶材料具有良好止血促凝效果,可为将纤维蛋白凝胶作为伤口辅料的进一步研究提供参考。

In this study, fibrinogen and thrombin were used to prepare fibrin-gels for obtaining a hemostasis materials. The fibrinogen solution and the thrombin solution were mixed with the proportion of 1 ∶1, 1 ∶3 and 1 ∶5. The SEM results show that, the fibrin-gels has a porous fiber network structure, and the pore size in 1 ∶3 fibrin-gels is the smallest one. The swelling degree measurements results indicate that the 1 ∶3 fibrin-gels possesses the largest swelling ratio. The procoagulant evaluation result show that the thrombus are much easy to form on the fibrin-gels surface due to the obviously activate platelets. The results of suckling rat wound experiment indicate that, the 1 ∶5 fibrin-gels promotes the healing of the wound. Above all, the prepared fibrin-gels in this work had good hemostasis ability. The obtained results are helpful for further research and development of fibrin-gels as wound dressing.