HPLC法同时测定抗癌平丸中8种成分

Simultaneous determination of eight constituents in Kangaiping Pills by HPLC

目的建立HPLC法同时测定抗癌平丸(半枝莲、肿节风、白花蛇舌草,等)中野黄芩苷、野黄芩素、反丁烯二酸、异嗪皮啶、落新妇苷、鸡屎藤次苷甲酯、车叶草苷酸、车叶草苷的含有量。方法该药物50%甲醇提取液的分析采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量0.9 m L/min;柱温30℃;检测波长210、238、335、344 nm。结果 8种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.89%~100.12%,RSD 0.79%~1.71%。结论该方法简便准确,重复性好,可用于抗癌平丸的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of scutellarin,scutellarein,funmaric acid,isofraxidin,astilbin,methyl paederosidate,asperulosidic acid and asperuloside in Kangaiping Pills [Scutellaria barbata D. Don,Sarcandra glabra（ Thunb.） Nakai,Hedyotis diffusa Willd,etc. ].METHODS The analysis of 50% methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent TCC18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 1% phosphoric acid flowing at 0. 9 m L/min in a gradient elution manner,and the detection wavelengths were set at 210,238,335,344 nm. RESULTS Eight constituents showed good linear relationships within their own ranges（ r 0. 999 0）,whose average recoveries were 96. 89%-100. 12% with the RSDs of 0. 79%-1. 71%. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Kangaiping Pills.