结直肠癌中RCE1的表达及意义

Influence of Rce1 Expression on Prognosis in Patients with Colorectal Cancer

目的探讨Rce1在结直肠癌组织中的表达差异及意义。方法采用免疫组化方法(IHC)检测237例结直肠癌患者癌组织及其匹配癌旁组织中Rce1的表达情况;进一步分析Rce1的表达情况与患者临床因素及预后的关系。结果 Rce1主要表达于细胞浆中,结直肠癌中Rce1表达率为54. 9%(130/237),明显低于癌旁组织78. 9%(187/237)(P <0. 05)。Rce1低表达与血液中癌胚抗原(CEA)表达水平、肿瘤浸润深度、有无淋巴结转移、肿瘤分期明显相关,差异具有统计学意义(P <0. 05);低表达Rce1的患者总体生存率及无瘤生存率均下降,对II和III期患者的进一步分析也表明,低表达Rce1的患者预后较差。Cox多因素分析结果显示Rce1是影响结直肠癌预后的独立危险因素(P <0. 05)。结论 Rce1在结直肠癌组织中表达缺失,且分析显示其表达缺失与结直肠癌侵袭性明显相关,低表达Rce1是患者预后不良的独立危险因素。

Objective To investigate the expression of Rcel in colorectal cancer （CRC） and its relationship with patients＇ clinicopathological characteristics and prognosis. Methods Clinical samples were collected from 237 CRC patients who had undergone curative resection, hnmunohistochemistry staining was conducted to detect the expression of Rcel in colorectal tumor tissues and adjacent non - tumor tissues. The correlation of Rcel expression with clinicopathological characterisfics and prognosis was analysed. Results Rcel is mainly expressed in cytoplasm. The positive rage of Rcel expression in tumor tissues was 54. 9% （ 130/237）, which was significantly lower than that in adjacent tissues 78.9% （ 187/237 ） （P 〈0. 05） ; Deficiency of Rcel expression was associated with CEA, depth of tumor invasion, node stage and TNM stage （ P 〈 0. 05 ） ; Decreasing expression of Rcel predicts poor prognosis for patients in both overall survival and disease - free survival. Further analysis of patients in stage Ⅱ and Ⅲ also indicated that patients with low Rcel had poor prognosis （ P 〈 0. 05 ）. Cox multivariate analysis showed that Rcel was an independent risk factor of CRC patient＇ s prognosis （ P 〈 0. 05 ）. Conclusion The outcomes of this study indicated that Rcel is markedly down - regulated in CRC tissues. Deficiency of Rcel expression may increase CRC cell invasion and independently predict an unsatisfactory prognosis in CRC patients.