市售香肠中亚硝酸盐含量的测定

Determination of Nitrite Content from the commercial Sausage

目的:通过测定市售香肠中亚硝酸盐的含量,检测计算香肠中亚硝酸盐含量是否在国家规定标准要求内,为相关的食品监管部门和消费者提供科学依据.方法:经过沉淀蛋白质和除去脂肪后的样品,在酸性体系中,样品中的NO2-在显色剂的作用下,发生偶联反应形成的紫红色产物在λmax的光下有最大吸收现象,根据工作曲线可以获得样品中亚硝酸盐含量.结果:样品检测结果表明:亚硝酸盐含量在1.178mg/kg^9.893mg/kg之间,均符合国家对亚硝酸盐含量的限量标准.结论:亚硝酸盐含量在安全值内,所测定的亚硝酸盐回收率范围在89.50%~109.59%,相对标准偏差在0.009%~0.133%之间,说明实验所采取的方法便捷有效,分光光度法测定准确,可靠.

Objective： To determine the nitrite content in the commercial sausages, whether the content are consistent with the ralated National Stardard, and it provides scientific basis for the food regulatory departments and consumers. Methods： After precipitation of proteins and fat removal of samples, in the acidic system, the NO2 in the sample interact with chromogenic reagent, under the light of λ max, the purplish red product formed by the coupling reaction has the maximum absorption phenomenon. According to the working curve, the nitrite content in the sample can be obtained. Results： The samples examination shows that the nitrite content is accord with the National Stardard in the range of 1.178mg/ kg-9.893mg/kg. Conclusion： The nitrite content is within the safe limit, the relative standard deviation of thirteen samples determination results were 0.009% -0.133% and recoveries were 89.50% -109.59%. It is available and effective way to get the experimental results by the experimental method, spectrophotometric determination is accurate and reliable.