高效液相色谱法测定不同乳制品中胆固醇的含量

Determination of cholesterol content in various dairy products with high performance liquid chromatography

目的测定乌鲁木齐市售牛乳、驼乳和驴乳中胆固醇含量,为居民膳食营养的选择提供参考。方法样品经皂化、提取、浓缩后,经C18柱分离,二极管阵列检测器205 nm检测。结果牛乳中胆固醇含量范围6.20~10.16 mg/100 g、均值8.39 mg/100 g,驼乳中胆固醇含量范围4.78~8.31 mg/100 g、均值6.86 mg/100 g,驴乳中胆固醇含量范围0.00~3.78 mg/100 g、均值1.39 mg/100 g。结论检测结果对于居民尤其是患有心血管等慢性病的居民选择乳制品有一定的参考意义。

Objective To determine the content of cholesterol in the milk of cow, camel and donkey sold in Urumqi market,and provide reference for the choice of dietary nutrition by residents. Methods Samples were detected by diode array detector at 205 nm wavelength after saponificating, extracting, concentrating and separating with C18 column. Results The cholesterol content was determinated in the milk of cow（6.20-10.16 mg/100 g, mean=8.39 mg/100 g）, camel（4.78-8.31 mg/100 g,mean=6.86 mg/100 g） and donkey（0.00-3.78 mg/100 g, mean=1.39 mg/100 g）. Conclusions The results are of certain reference for the resident, especially those with chronic diseases like cardiovascular disease etc. to select dairy products.