半乳糖凝集素3低表达对人胃癌MGC-803细胞增殖的影响

Influence of galectin-3 low expression on proliferation of MGC-803 cells

目的探讨半乳糖凝集素3(galectin-3)表达抑制对人胃癌细胞系MGC-803细胞增殖产生的影响。方法将胃癌细胞系MGC-803细胞分为3组,siRNA干扰组(转染靶向galectin-3的特异siRNA),空白对照组(只加转染试剂)和阴性对照组(转染非特异性的siRNA)。用Western blotting法检测转染后细胞中galectin-3蛋白的抑制效率,应用MTT法、集落形成实验检测galectin-3基因低表达对MGC-803细胞增殖的影响;用Western blotting和免疫组织化学法检测细胞周期蛋白D1(cyclin D1)和增殖细胞核抗原(PCNA)的表达情况。结果 siRNA干扰组细胞中galectin-3蛋白的表达与阴性对照组和空白对照组相比明显降低(P<0.05);细胞集落形成实验和MTT显示,siRNA干扰组细胞的增殖能力与阴性对照和空白对照组细胞比较显著降低(P<0.05);cyclin D1和PCNA蛋白表达均为siRNA干扰组细胞表达显著低于两对照组(P<0.05)。结论 Galectin-3低表达可抑制胃癌MGC-803细胞的增殖能力,这可能与抑制cyclin D1和PCNA的表达有关。

Objective To investigate the influence of galectin-3 suppression on the proliferation of human stomach carcinoma MGC-803 cells by down-regulating galectin-3 expression using RNA interference technique. Methods The MGC-803 cells were divided into three groups. The cells transfected with siRNA targeted galectin-3 was regarded as si-RNA interference group, the cells transfected with scrambled siRNA was taken for negative control and those with lipofectine TM2000 alone was blank control. Western blotting was applied to detect the suppression rate of galectin-3. The growth of the ceils was assessed by MTT and colony formation assays. Western blotting and immunohistochemistry were used to detect the expression of cyclin D1 and proliferationg cell nuclear antigen（PCNA）. Results The expression of galectin-3 was significantly inhibited in the experiment cells group when compared with those of the blank ceils group and negative ceils group （P 〈 0. 05 ）. MTT and colony formation assays showed that proliferation of the experiment cells group decreased significantly （ P 〈 0.05）. The expression of eyclin D1 and PCNA decreased compared with the negative control group and the blank control group （P 〈 0. 05）. Conclusion Galectin-3 low expression inhibits the proliferation of M GC-803 cells which might associate with the suppression of cyclin D1 and PCNA expression.