人脂肪干细胞的分离、培养及鉴定

Isolation,Culture and Identification of Human Adipose-derived Stem Cells

目的:探讨胶原酶消化法从人脂肪组织中提取脂肪干细胞(adipose-derived stem cells,ADSCs)的可行性。方法:采用胶原酶消化法将真空抽脂术抽取的新鲜脂肪组织进行消化分离,获取ADSCs并进行培养,用流式细胞术鉴定ADSCs的表面特异性标志物CD34、CD44、CD45、CD105的表达。结果:利用胶原酶消化法可成功提取ADSCs,其标志物CD44和CD105表达阳性,CD34为低表达, CD45表达为阴性。结论:通过胶原酶法可成功提取人ADSCs。

Objective： To investigate the isolation, culture and identification of the adipose-derived stem cells （ADSCs） from human adipose tissue in vitro by collagenase digestion method. Methods： The subcutaneous adipose tissue was obtained from caesarean section, and then was digested with 0.2% I collagenase under the aseptic condition. The isolated ADSCs were cultured and the morphology and proliferation charaeteristics were observed. ADSCs were identified by the expression of sin-face specific markers CD34, CD44, CD45 and CD105 with flow cytometry. Results： ADSCs were successfully isolated and cultured. The cell surface markers CD44 and CD105 were positively expressed, CD34 was weakly expressed, and CD45 were negatively expressed. Conclusion ： The human ADSCs can be successfully isolated by collagenase digestion method.