甘菊CBL基因的克隆与表达分析

Cloning and expression analysis of CBL genes from Chrysanthemum lavandulifolium

基于已建立的甘菊ESTs文库,检索到了CBL基因4个不同的EST序列;采用RACE技术获得了它们的全长序列,并命名为ClCBL1-4。将甘菊(Chrysanthemum lavandulifolium)ClCBL与拟南芥(Arabidopsis thaliana)的CBL进行多重序列比对发现,它们都含有4个结合钙离子的EF-hand结构域,且ClCBL1在N端有一个豆蔻酰化序列(MGCXXSK/T)。采用RT-PCR技术,分析了甘菊中CBL基因的表达与不同逆境胁迫的关系,表明甘菊CBL基因的表达在冷、干旱、热、盐及ABA胁迫中可被不同程度地诱导,而在非逆境胁迫条件下,甘菊CBL基因在根、茎、叶及花等不同器官中均有表达,且表达量相对一致。这些结果说明该家族基因参与了植物非生物逆境胁迫的抵御。

Four ESTs encoding CBLfromChrysanthemum lavandulifolium were identified in our previous study.Four CBLgenes were isolated fromC.lavandulifoliumand named after ClCBL1-4 using RACE method.The multiple sequence alignment analysis of Arabidopsis thaliana and C.lavandulifolium CBL sequences suggested that all of the ClCBL1-4 proteins coding sequence contained four EF-hand motifs and the ClCBL1 protein coding sequence had N-terminal myristoylation sequence.The expression patterns of ClCBLs were analysis under salt,drought,cold,heat and ABA treatments as well as under normal conditions in different developmental stages.The results suggested that ClCBLs might play important roles in C.lavandulifolium's responses to abiotic stresses because ClCBLs can be induced under different stresses.