血清p53抗体是消化系肿瘤新的标志物

Serum p53 protein is a new marker of digestive neoplasms

目的 探讨血清p5 3抗体对消化系肿瘤的诊断价值。方法 应用ELISA法检测了食管癌、胃癌、肝癌患者血清中p5 3抗体的浓度。结果 血清p5 3抗体浓度在肿瘤患者和良性疾病对照组中分别为 46 .9pg/mL和 18.6pg/mL(P <0 .0 5 )。以对照组 X± 2S为界值 ( 2 1.3pg/mL) ,则肿瘤组血清p5 3抗体阳性率为 2 1.4%,而对照组则为 5 .6 %(P <0 .0 5 )。肿瘤伴远处转移者p5 3抗体阳性率高于无转移者 (P <0 .0 0 5 ) ,手术后p5 3抗体浓度显著下降 (P <0 .0 5 )。血清p5 3抗体与CEA显著相关 (P <0 .0 0 5 )。血清p5 3抗体对消化系肿瘤诊断的敏感性、特异性、准确性分别为 2 1.4%、94.4%、5 0 %。结论 血清p5 3抗体浓度在消化系肿瘤中异常升高 ,是特异性的肿瘤标志物。

Aim To investigate the value of serum p53 protein in digestive neoplasms.Methods The concentrations of p53 protein were detected by an enzyme linked immunosorbent assay in 36 cases of esophageal cancer,68 cases of gastric cancer and 18 cases of hepatocellular cancer.Results The mean serum concentration of p53 protein in patients and negative controls were 46.9 pg/mL and 18.6 pg/mL respectively,and had significant difference between them(P<0.05).An arbitrary was adopted to cut off value for the serum p53 protein concentration of 21.3 pg/mL,which corresponded to a value 2S above the mean vale from the controls,the positive rates of serum p53 protein in patients was 21.3%(26/122),and was significantly higher than that in controls (5.6%,2/36)(P<0.05).The serum p53 concentration was positive in 16 out of 42 patients with carcinomas of distant metastases,but only 10 of 80 patients with carcinomas without metastases (P<0.005).The mean serum p53 protein concentration after tumor resection significantly decreased compared with the preoperative concentration (P<0.05).Serum p53 protein correlated with tumor marker CEA(P<0.005).The sensitivity,specificity and accuracy of diagnosing digestive neoplasms with p53 antibody were 21.4%?94.4%?50% respectively.Conclusions The serum concentration of p53 protein arises in digestive carcinomas and is a new tumor marker.