流式细胞仪BrdU/DNA双参数分析法测定急性白血病细胞周期及其临床意义

FLOW CYTOMETRIC MEASUREMENTS OF CELL CYCLE KINETICS BY BROMODEOXYURIDINE(BRDU)/DNA BIP- ARAMETER ANALYSIS AND THEIR PROGNOSTIC VALUE IN ACUTE LEUKEMIA

研究白血病细胞的增殖动力学,探讨其与临床的关系。采用流式细胞仪（FCM）BrdU/DNA双参数分析法测定39例急性白血病骨髓细胞周期的各时相细胞比例及S期细胞的时限（Ts）,其中ANLL26例,ALL13例;初治31例,复发8例（ANLL、ALL各4例）。结果显示,急性白血病骨髓细胞S期比例显著低于正常,初治及复发白血病患者骨髓S期细胞比例之间相差不显著;复发白血病患者骨髓细胞的Ts比初治白血病患者及正常骨髓细胞的Ts都短。提示白血病细胞处于较正常低增殖状态;Ts时限短提示白血病细胞再生迅速,与急性白血病复发关系密切;Ts时限短为白血病预后不良因素之一。

To study cell cycle kinetics of acute leukemia cells and to find out its relationship with clinical treatment outcomes, flow cyto-metric measurements of cell cycle kinetics, especially percentage of S-phase cells (S%) and duration of S-phase(Ts), were made in bone marrow cells of 39 patients with acute leukemia, including 26 cases of ANLL and 13 cases of ALL. Among them 31 cases were at diagnosis and 8 were in relapes. Bromodeoxyuridine(BrdU) / DNA biparameter analysis was used, in which cells were stained with propidium iodide(PI) to estimate total cellular UNA content and a monoclonal antibody against BrdU as a probe for BrdU incorporated into DNA. The results showed that the mean S% of bone marrow cells was significantly lower than that of normal controls, while there was no significant difference between S% of patients at diagnosis and of those in relapse. However, the mean Ts was not significantly different in patients and in normal subjects, but Ts of patients in relapse was significantly shorter than that of the patients at diagnosis and that of normal controls. It is suggested that the proliferation rate of leukemic cells is lower than normal. Shorter Ts indicates faster regrowth of leukemic cells, which is closely related to relapse.