内江猪、伍隍猪生化遗传及细胞遗传的研究:Ⅰ血清乳酸脱氢酶同工酶的分离测定

Study on Biochemical Genetics and Cell Genetics in Neijiang and Wuhuang Pig Ⅰ: Determination of Lactic Dehydrogenase (LDH)

采用聚丙烯酰胺凝胶盘状电泳法,在完全相同条件下,分别测定了三月龄和六月龄的内江猪、伍隍猪的血清乳酸脱氢酶(LDH)同工酶,并与相应月龄的体尺、体重等进行分析。结果表明:内江猪和伍隍猪血清LDH同工酶表现出五条正常酶带。由正极到负板依次为LDH_1,LDH_2,LDH_3,LDH_4,LDH_5。三月龄时,内江猪和伍隍猪血清LDH同工酶活力均值(％)的顺序是LDH_1>LDH_5>LDH_3>LDH_4>LDH_2,组间差异不显著(P>0.05)。六月龄时,两组LDH同工酶活力均值(％)顺序为LDH_1>LDH_5>LDH_4>LDH_3>LDH_3,组间差异也不显著(P>0.05)。内江猪的LDH同工酶三月龄与六月龄相比无显著差异(P>0.05)。而伍隍猪的LDH_2在三月龄时极显著的高于六月龄(P<0.01),其余差异不显著。血清LDH同工酶与体尺体重等的关系,在内江猪与伍隍猪之间和三月龄与六月龄之间均未发现什么规律性。

Under same condition, the isozymes of serum lactic dehydrogenase (LDH) were separated and determined in Neijiang and Wahuang pig(a-month-old and 6-month-old) with the method of disc polyacrylamide gel electropho-resis. The correlative analysis between the isozymes and body weight and body measurements etcwere done. The results indicated that serum LDH isozymes had five normal enzyme bands,which were LDH_1 LDH_2 LDH_3 LDH_4 LDH_5 from positive to negative electrode. The serum LDH isozymes activity showed LDH_1>LDH_5>LDH_3>LDH_4>LDH_2 and LDH_1>LDH_5>LDH_4>LDH_3 >LDH_2 for age groups of three and six months, respectively. There was no significant difference between the Neiiiang pig and wuhuang pig(p>0.05). Difference was not significant in LDH isozymes between the 3-month-old Neijiangand the 6-monfh-old Neiiiang (P>0.05). The aveLage value of LDH_2 in 3-month-old Wuhuang pig was distinctly higher than that of LDH_2 in 6-month-old Wuhuang pig(p<0.01), but the others were not (P>0.05). The regularity of relationships between LDH isozymes and body weight and body measurements have not been found.