蜂蜜中蛋白质及氨基酸含量的测定

The Content Determination of Protein and Amino acid in Honey

分别采用考马斯亮蓝法和茚三酮显色法测定6个蜂蜜样品中的蛋白质和氨基酸含量。实验结果表明，100g蜂蜜样品中，蛋白质和氨基酸的含量分别为0.044～0.391g和0.031～0.055g，表明不同蜂蜜样品中的蛋白质和氨基酸的含量各不相同。蜂蜜样品中蛋白质和氨基酸测定时的相对标准偏差分别为0.666%～6.447%和0.601%～1.306%，平行样品间的测定误差较小。

The methods pf Bradford and Ninhydrin colorimetric were chosen to determine the contentsof protein and amino acid of six samples of honeys.Results showed that the contents of protein and aminoacid in100g honey samples were0.044～0.391g and0.031～0.055g,the contents of them in six honeysamples were different from each other.The relative standard deviations was0.666%～6.447%for proteintest and0.601%～1.306%for amino acid test.All deviations among the parallel samples were small.