白芍总苷对应激大鼠的行为和下丘脑神经内分泌的影响

Effect of Total Glucosides of White Paeony Capsules on Psychological Ethology and Hypothalamic Neuroendocrine System of Stress Rats

目的观察白芍总苷对应激大鼠行为和神经内分泌的影响,探讨其部分作用机制。方法雄性SD大鼠取10只作为正常对照组,其余40只采用不可预见性中等刺激建立应激大鼠模型,随机分为模型对照组、氟西汀(5.0 mg·kg^(-1))组和白芍总苷大、小剂量(66,33 mg·kg^(-1))组,每组10只。连续给药3周后,使用自主活动仪测定大鼠水平和垂直活动变化;采用悬尾实验观察大鼠情绪变化;采用苏木精-伊红染色观察肾上腺形态学变化,放射免疫法测定皮质酮和促肾上腺皮质激素(ACTH)的含量,实时-聚合酶链反应(RT-PCR)法检测下丘脑促肾上腺皮质素释放激素(CRH)mRNA的表达。结果与模型对照组比较,白芍总苷大剂量组水平和垂直活动增加(P<0.05);不动时间减少(P<0.05),肾上腺指数下降,血清皮质酮和ACTH下降(P<0.05);下丘脑CRH mRNA的表达下降(P<0.05)。结论白芍总苷能纠正应激大鼠行为和内分泌紊乱,可能是通过降低皮质酮水平和下丘脑CRH mRNA的表达,调整紊乱的下丘脑-垂体-肾上腺轴活动实现。

ObjectiveTo observe the effects of total glucosides of white paeony(GWP)on psychological ethology and neuroendocrine system of stress rats.MethodsTen male Sprague Dawley rats were treated as normal control group,the others(n=40)were constructed by isolated custody and chronic unpredictable stimuli to build the model of stress rats.Stress rats were randomly divided into4groups:model control group,GWP high dose group and low dose(66,33mg·kg-1)group,fluoxetine(5.0mg·kg-1)group,each group had10rats.The changes of rat behavior were monitored by locomotor activity system.The change of emotion was observed by tail suspension test.The pathology change of adrenal was observed by HE staining.The levels of CorT and ACTH in serum were measured by radioimmunoassay.The expression of CRH mRNA of hypothalamic were detected by RT PCR.ResultsThe activity of the level scoring and vertical scoring of GWP high dose group was obviously improved as compared to that in model control group,while static time was significantly shortened,Adrenal index of GWP groups were increased significantly(P<0.05),thymus index of the treatment group was declined(P<0.05),CorT and ACTH content were decreased(P<0.05)and hypothalamus CRH mRNA of GWP group was decreased(P<0.05).ConclusionThe improvement of activity and related hormone anomalous changes were observed in rats treated with GWP.The mechanism of its actions might be involved adjusting the endocrine function,decreasing CorT and ACTH content and the expression of hypothalamic CRH mRNA of stress rats.