血和尿钙卫蛋白、IL-18及血清肌酐对脓毒症小鼠并发急性肾损伤的早期诊断价值研究

The study of the diagnostic value of Calprotectin,IL-18 indexes in the serum and urine and Cr in the serum in the early stage of mouse acute renal injury with sepsis

目的 :研究血和尿钙卫蛋白和IL-18等指标对脓毒症小鼠并发急性肾损伤的早期诊断价值。方法 :将48只ICR雄性小鼠随机分成0h、6h、12h和24h亚组,每亚组12只小鼠。0h组为对照组,注射生理盐水,6只立即取血液样本,6只收集24h尿液;其余3组小鼠均接受尾静脉注射细菌脂多糖(Lipopolysaccharide,LPS),5mg/kg,分别于6h、12h和24h时间点收集尿液并取血液样本。所有血清标本检测钙卫蛋白、IL-18及肌酐;尿标本检测钙卫蛋白、IL-18。使用SNK检验。结果:血和尿钙卫蛋白、IL-18及血肌酐等指标在注射LPS后6小时开始上升,除血IL-18 P值为0.02外,其余各组均P<0.001。结论:血钙卫蛋白和IL-18指标不能预测脓毒症小鼠并发AKI;而尿钙卫蛋白和IL-18指标可用于脓毒症合并AKI的早期诊断和预测,且尿钙卫蛋白具有更早的预测价值。

Objective:To study the diagnostic value of Calprotectin,IL-18 indexes in the serum and urine and Cr in the serum in the early stage of mouse acute renal injury with sepsis.Methods:48 ICR male mice were randomly divided into 0h,6h,12h and 24h subgroups,with 12 mice for each subgroup.The 0h group was used as the control group,which was injected with saline.Blood samples from 6 mice were taken immediately and 6 urine was collected in 24h.The other three groups were injected by lipopolysaccharide(LPS),5mg/kg via tail vein.From them urine was collected at 6h,12h and 24h respectively.All serum samples were measured for calprotectin,IL-18 and creatinine indexes.Urine specimens were examined for Calprotectin and IL-18 indexes.By using SNK test,P<0.05 was considered as the statistically significant difference.Results:Hematuria Calprotectin,IL-18 and serum creatinine indexes were increased at 6 hours after injection of LPS.P value:except serum IL-18 as 0.02,the others were less than 0.001.Conclusions:Hematuria Calprotectin and IL-18 indexes cannot be used to predict mouse AKI with sepsis,while urinary Calprotectin and IL-18 indexes can do.Besides,urinary calprotectin has a better value of early prediction.