脂肪干细胞培养研究进展

Cultivation of adipose-derived stem cells: research progress

脂肪干细胞因其诸多优势被广泛应用到再生医学中,高质量的脂肪干细胞是其应用到再生医学上的前提。而培养条件和增殖分化相关的分子调控是得到高质量脂肪干细胞的关键。文章主要对培养条件如培养方式、培养液的种类、血清、氧浓度、细胞接种密度、细胞代数等以及增殖分化相关分子调控如生长因子、MicroRNAs、Wnt/Notch信号通路等方面对脂肪干细胞生物学特性的影响进行综述。

Adipose-derived stem cells( ASCs) as potential seeded cells have been widely used in regenerative medicine because of their many advantages. High-quality ASCs is an important premise for their application in regenerative medicine. However,culture conditions and molecular regulation of proliferation and differentiation are the key to obtaining high-quality ASCs. In this review,the effects of culture conditions such as culture mode,culture medium type,serum,oxygen concentration,cell inoculation density,cell passages,and molecular regulation of proliferation and differentiation such as growth factor,microRNAs,Wnt/Notch signaling pathway on the biological characteristics of ASCs were reviewed in order to provide theoretical basis for obtaining high-quality ASCs.