慢性HIV感染者外周血T细胞CD28、CD160表达水平的研究

Research on the expression of CD28 and CD160 in patients with chronic HIV infection

目的探讨CD28、CD160在慢性人类免疫缺陷病毒(HIV)感染者体内的表达及其临床意义。方法选取2016年1月至2017年1月收治的50例HIV感染患者,作为观察组,并征集50例健康志愿者,作为对照组。观察并记录所有研究参与者一般资料、CD4+、CD8+T细胞上CD160及CD28的表达及初始T细胞(TN)、中央记忆T细胞(TCM)、效应记忆T细胞(TEM)、终末效应记忆T细胞(TEMRA)亚群上CD160表达的百分比及平均荧光强度(MFI)、两种细胞上病毒载量,并分析CD28及CD160的表达水平与患者CD4+T细胞计数和病毒载量的相关性。结果随着CD4+CD160的表达增加,CD4+T细胞呈下降趋势,两者呈负相关(r=-0.561,P<0.05),CD8+T细胞数量呈上升趋势,两者呈正相关(r=0.619,P<0.05),而HIV-RNA拷贝数量随着CD4+T细胞上、CD8+T细胞上CD160表达的增加而增加,两者呈正相关(r=0.684,P<0.05、r=0.459,P<0.05);随着CD4+T细胞上CD28的表达增加,CD4+、CD8+T细胞计数呈上升趋势,两者呈正相关(r=0.621,P<0.05、r=0.527,P<0.05),而HIV-RNA拷贝数量随着CD4+、CD8+T细胞上CD28表达的增加而降低,两者呈负相关(r=-0.634,P<0.05、r=-0.582,P<0.05);两组患者CD8+T细胞CD160在TEMRA亚群表达阳性率及MFI差异无统计学意义(P>0.05),观察组患者CD8+T细胞CD160在TN、TCM、TEM亚群表达阳性率及MFI均高于对照组,差异有统计学意义(P<0.05)。结论慢性HIV感染患者中CD28表达有所减少,而CD160表达有所增加,可能与HIV患者CD4+T、CD8+T细胞减少有关,其中CD160主要影响记忆性CD8+T细胞。

Objective To investigate the expression and clinical significance of CD28 and CD160 in patients with chronic HIV infection.Methods 50 patients with HIV from January 2016 to January 2017 were selected as the observation group,and 50 healthy volunteers were recruited as control group.Observe and record general information of all participants,the expression of CD28,CD160 in CD4^+and CD8^+T cells,initial T cells(TN),the expression of CD160 in central memory T cells(TCM),effector memory T cells(TEM),end effector memory T cells(TEMRA),mean fluorescence intensity(MFI),viral load of two kinds of the cells,analyze the correlation between the expression level of CD28 and CD160 and CD4^+T cell count and viral load.Results With the increase of CD160 expression of CD4^+T cells,CD4^+T cells showed a downward trend,there is a negative correlation between them(r=-0.561,P<0.05),CD8^+T cell number is on the rise,there is a positive correlation between them(r=0.619,P<0.05),and HIV-RNA copy number increased with the increase of CD160 expression on CD4^+T cells and CD8^+T cells,both positive(r=0.684,P<0.05,r=0.459,P<0.05);with the increase of CD28 cells on the expression of CD4^+T,CD4^+,CD8^+T cells showed a rising trend,there is a positive correlation between them(r=0.621,P<0.05,r=0.527,P<0.05,HIV-RNA)and the copy number decreased with the increase of the expression of CD28 and CD4^+T on CD8^+T cells,there is a negative correlation between them(r=-0.634,P<0.05,r=-0.582,P<0.05).There was no significant difference in the positive rate of expression in TEMRA subgroup and MFI of CD160 in CD8^+T cell in two groups(P>0.05).The positive rate and MFI of CD8^+T cell CD160 in TN,TCM and TEM subgroups in observation group were significantly higher than those in control group(Tcm),with statistical significance.Conclusion The expression of CD28 in patients with chronic HIV infection is decreased,and the expression of CD160 is increased,which may be related to the decrease of HIV CD4^+T and CD8^+T cells,in which CD160 mainly affects the memory CD8^+T.