小麦开花后籽粒多组分营养物质积累动态分析

Dynamic Analysis of the Accumulation of Multi-component Nutrients in Grains after Wheat Flowering

为探究小麦开花后籽粒中多组分营养物质积累的动态规律及其相互间的关系,本研究以六倍体栽培小麦中国春和贵紫1号为材料,对开花后10、15、20、25、30、35、40d的籽粒总类黄酮、总酚、植酸、无机磷、总淀粉、氨基和可溶性蛋白质含量及单粒重进行了动态测定及单因素方差(One-Way ANOVA)、相关性网络(Correlation-based network analysis,CAN)和斯皮尔曼秩相关(Spearmanp’s Rho Correlation)等分析。结果表明,在籽粒发育过程中,氨基、可溶性蛋白质以及无机磷含量逐渐降低,淀粉含量升高后逐渐趋于稳定,总酚含量先降低后升高,总类黄酮含量及单粒重先升高后降低。2个小麦品种籽粒中各营养物质含量及单粒重的动态变化趋势相似,但各营养物质含量的高低及其差异显著性却各有不同。不同阶段的单粒重与对应阶段营养物质的含量存在不同程度的相关性,即使是同一种营养物质,在籽粒发育的不同阶段与单粒重的相关性也不尽相同。

In order to explore the dynamic law of the accumulation of multi-component nutrients in the grains of wheat after flowering and the relationship between them,we used the hexaploid-cultivated wheats(Chinese spring and Guizi no.1)as materials.The dynamics measurement of main nutritional components content such as the grain total flavonoids,total phenol,phytic acid,inorganic phosphorus,total starch,amino and soluble protein content and single grain weight at 10,15,20,25,30,35 and 40 days after flowering were measured and related data were analyzed by one-way ANOVA,Correlation-based network analysis(CAN)and Spearmanp’s Rho Correlation.The results showed that during grain development,the content of amino group,soluble protein and inorganic phosphorus gradually decreased,the content of starch gradually became stable after increasing,the content of total phenol first decreased and then increased,and the content of total flavonoids and single grain weight first increased and then decreased.The dynamic change trend of each nutrient content and single grain weight in the grains of the two wheat varieties were similar,but the levels of each nutrient content and their significant differences were different.There were different degrees of correlation between single grain weight at different stages and the content of nutrients at corresponding stages.Even for the same nutrient substance at different stages of grain development,it has different correlations with single grain weight.