CIK与NK细胞联合应用对肿瘤细胞的杀伤性研究

Study on the Killing Effect of CIK Combined with NK Cells on Tumor Cells

目的:探讨CIK与NK细胞联合应用对肿瘤细胞的杀伤功能。方法:选取2018年1月至7月收治的10例肿瘤患者作为研究对象,采集患者外周血,分离单个核细胞,分别诱导扩增制备成CIK、NK细胞。分别检测CIK、NK及两者联合应用对肿瘤细胞K562的杀伤率。结果:在效靶比5∶1、10∶1、20∶1条件下,CIK/NK 1∶1联合组对K562肿瘤细胞的杀伤率分别为(48.1±1.9)%、(61.3±2.5)%、(81.2±2.6)%,高于CIK组的(31.2±1.5)%、(42.7±2.1)%、(61.3±2.2)%和NK组的(35.4±1.7)%、(46.2±2.3)%、(63.5±2.1)%,各组间差异有统计学意义(P <0.05)。结论:CIK、NK细胞联合应用的效果优于单独使用,证实两者的共同作用可以提高对肿瘤细胞的杀伤毒性,在临床中可联合两种细胞应用于肿瘤的免疫治疗。

Objective:To analyze the killing function of CIK combined with NK cells on tumor cells.Method:Ten patients with tumors admitted from January to July 2018 were enrolled.The peripheral blood of patients was collected and mononuclear cells were isolated and induced to form CIK and NK cells.The killing rate of tumor cells K562 was detected by CIK,NK and the combination of the two.Result:At the effective target ratio of 5∶1,10∶1,20∶1,the killing rate of K562 tumor cells in the CIK/NK 1∶1 combination group was(48.1±1.9)%,(61.3±2.5)%,(81.2±2.6)%,respectively.higher than(31.2±1.5)%,(42.7±2.1)%,(61.3±2.2)%of the CIK group and(35.4±1.7)%,(46.2±2.3)%,(63.5±2.1)%of the NK group,the difference between the groups was statistically significant(P<0.05).Conclusion:The combined effect of CIK and NK cells is better than that of single use.It is proved that the combined effect of the two can improve the killing toxicity of tumor cells.In clinical,two cells can be combined for tumor immunotherapy.