一株纤维素酶产生菌的筛选与产酶特性研究

Optimization of Enzyme Production Conditions for A Cellulase-producing Strain

纤维素降解菌在纤维素物质的降解和资源化利用中具有重要作用。利用传统微生物学方法,从腐烂秸秆中筛选出一株高产纤维素酶的真菌QL06,通过形态学特征与ITSDNA序列同源性分析,该株菌为烟曲霉(Aspergillusfumigatus),以单因素试验与正交试验对产酶条件进行优化。结果表明,QL06培养84 h所产CMC酶活和生物量均最大,以麸皮+葡萄糖为碳源、酵母膏为氮源时有利于QL06产酶;在接种量体积分数为8%、麸皮+葡萄糖质量分数2%、酵母膏质量分数1.5%、初始pH值为4.0,培养温度40℃时,CMC酶活力达5.41 U/mL,具有进一步开发利用的价值。

Cellulose-degrading bacteria play an important role in the degradation and resource utilization of cellulosic materials.In this paper,a high-yield cellulase-producing fungus QL06 was screened from decayed straw by traditional microbiological method.The morphological characteristics and ITS DNA sequence homology analysis showed that the strain was Aspergillus fumigatus with single factor.The enzyme production conditions were optimized by experiments and orthogonal experiments.The results showed that the activity and biomass of CMC produced by QL06 cultured for 84 h were the highest.When Bran+glucose was used as carbon source and yeast extract was used as nitrogen source,it was beneficial to QL06 production enzyme;inoculation amount volume fraction was 8%,bran+glucose mass fraction 2%,yeast extract mass fraction 1.5%,initial pH 4.0,CMC enzyme activity reached 5.41 U/mL at a culture temperature of 40℃,which has further development and utilization value.