摘要
建立了花生芽和葡萄籽微波超声波联用提取白黎芦醇及其苷的HPLC测定方法。采用高效液相色谱进行测定,色谱柱为迪马C18,流动相为乙腈-水溶液,梯度洗脱,流速1.0ml/min,柱温为30℃,检测波长为306nm,进样量为20μl。白黎芦醇在0.343~15.15×103μg/ml范围内呈良好的线性关系,相关系数R2=0.9967;白藜芦醇苷在110.712~1774.23×103μg/ml范围内线性关系良好,相关系数R2=0.9993。该方法检测快速、简便、重现性好,适用于花生芽和葡萄籽中白藜芦醇及其苷的定量分析。
Objective to establish a method for the determination of resveratrol and polydatin in peanut bud and grape pip extract. High performance liquid chromatography (HPLC) was used. The chromatographic column was Di Ma C 18 , the mobile phase was acetonitrile water solution, gradient elution, flow rate 1 ml/min, column temperature 30℃, 306 nm and 20 μl. The linear range of resveratol showed good linear relationship 0.343-15.15×10 3 μg /ml, R 2 = 0.9967. The linear range of polydatin showed good linear relationship 110.712-1774.23 × 10 3 μg / ml, R 2 = 0.9993. The method is rapid, simple and reproducible, and is suitable for quantitative analysis of resveratrol in peanut sprouts and grape seeds.
作者
马密霞
秦宁
闵清
胡文祥
MA Mixia;QIN Ning;MIN Qing;HU Wenxiang(School of Chemical and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, Hubei, China;Smart City College, Beijing Union University, Beijing 100101, China;School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, Hubei, China;Jingdong Xianghu Microwave Chemistry Union Laboratory, Beijing Excalibur Space Military Academy of Medical Sciences, Beijing 101601, China)
出处
《化工学报》
EI
CAS
CSCD
北大核心
2019年第A01期124-129,共6页
CIESC Journal
基金
北京市创新基金项目(Z080104024508111)
关键词
微波超声波
萃取
白藜芦醇
药物
色谱
浸取
microwave ultrasonic wave
extraction
resveratrol
pharmaceuticals
chromatography
leaching
