摘要
本实验主要探究发酵乳杆菌AR497对DSS诱导的小鼠炎症性肠病的影响。发酵乳杆菌AR497在低pH、高胆盐浓度、高渗透压等极端条件下仍具有良好的生长能力,对大部分抗生素敏感;在C57BL/6J小鼠中研究其对由葡聚糖硫酸钠(DSS)诱导的结肠炎的缓解作用,发酵乳杆菌AR497处理后抑制小鼠体重减少,降低疾病活动指数,上调紧密连接蛋白基因Claudin 3,ZO-1和E-cadherin 1的表达。实验结果表明发酵乳杆菌AR497具有优良的生物学特性并可通过保护肠道屏障从而缓解DSS诱导的结肠炎。
The main purpose of this study was to explore the effects of Lactobacillus fermentum AR497 on DSS-induced inflammatory bowel disease in mice. Lactobacillus fermentum AR497 had good growth ability under extreme conditions such as low pH, high bile salt concentration and high osmotic pressure, and was sensitive to most antibiotics. The effects of Lactobacillus fermentum AR497 on colitis induced by dextran sulfate sodium (DSS) in C57BL/6J mice were investigated. Lactobacillus fermentum AR497 treatment could inhibit the body weight loss, reduce the disease activity index and up-regulate the expression of the tight junction protein genes Claudin 3, ZO-1 and E-cadherin 1. The experimental results showed that Lactobacillus fermentum AR497 had excellent biological properties and could alleviate DSS-induced colitis by protecting the intestinal barrier.
作者
陈燕
林祥娜
王光强
熊智强
艾连中
夏永军
CHEN Yan;LIN Xiangna;WANG Guangqiang;XIONG Zhi qiang;AI Lianzhong;XIAYongjun(School of Medical Instrument and Food Engineering, University of Shanghaifor Science and Technology,Shanghai Engineering Research Center of Food Microbiology, Shanghai 200093)
出处
《工业微生物》
CAS
2019年第2期37-42,共6页
Industrial Microbiology
基金
国家自然科学基金面上项目(31771956)
上海市大学生创新创业训练计划项目(XJ10252258)
沪江基金研究基地专项(D15012)
关键词
发酵乳杆菌
结肠炎
肠道屏障
紧密连接蛋白
Lactobacillus fermentum
colitis
intestinal barrier
tightjunction protein
