摘要
为了优化微波辐射预处理提取地菍总黄酮的工艺及挖掘其生物活性,在单因素试验基础上,采用正交实验(L_(18)(3~7))确定总黄酮提取最佳工艺,参照CLSI标准测定了总黄酮提取物对金葡菌等5种细菌的抑菌活性。结果显示,地菍(根、茎)总黄酮提取的最优工艺为:微波功率420 W,汽化剂用量0 mL,微波时间120 s,水浴温度60℃,水浴时间90 min,乙醇浓度45%,固液比为1:40,黄酮提取率为34.755 mg·g^(-1);叶的最优提取工艺为:微波功率630 W,汽化剂用量0 mL,微波时间30 s,水浴温度60℃,水浴时间60 min,乙醇浓度60%,固液比为1:20,黄酮提取率为25.431 mg·g^(-1);果的最优提取工艺为:微波功率350 W,汽化剂用量0 mL,微波时间30 s,水浴温度80℃,水浴时间60 min,乙醇浓度60%,固液比1:50,黄酮提取率为39.952 mg·g^(-1),较传统水浴提高了46.366%。抑菌实验显示根茎总黄酮提取物对铜绿假单胞菌(标准)、铜绿假单胞菌(耐药)、金黄色葡萄球(标准)、金黄色葡萄球菌(耐药)、大肠杆菌的MIC分别为8 mg·mL^(-1)、8 mg·mL^(-1)、8 mg·mL^(-1)、8 mg·mL^(-1)、16 mg·mL^(-1),叶总黄酮对前4种菌的MIC分别为8 mg·mL^(-1)、16 mg·mL^(-1)、16 mg·mL^(-1)、8 mg·mL^(-1)。实验表明,微波辐射预处理对地菍总黄酮的提取具有指导意义。地菍总黄酮抑菌活性值得进一步研究。
In order to optimize the extraction technology of total flavonoidsby microwave irradiation pretreatment and explore its bioactivity from Melastoma dodecandrum. The L18 (37) Orthogonal experiment based on the results of one-factor experiments was used to optimize the best extraction process of the total flavonoids;The activity of total flavonoids against 5 kinds of bacteria was tested according to the CLSI standard.The results indicated that the optimal conditions from Melastoma dodecandrum(root and stem) were as follows: microwave power was 420 W, mass of vaporization solvent was 0 mL, microwave irradiation time was 120 s, water bath temperature was 60 ℃, water bath time was 90 min, ethanol concentration was 45 %and the solid to liquid ratio was 1:40 (g·mL^-1). under this condition, the yield of flavonoids was 34.755 mg·g^-1. The optimal conditions of the leaf were as follows: microwave power was 630 W, mass of vaporization solvent was 0 mL, microwave irradiation time was 30 s, water bath temperature was 60℃, water bath time was 60min, ethanol concentration was 60 %and solid to liquid ratio was 1:20 (g·mL^-1). under this condition, the yield of flavonoids was 25.431 mg·g^-1. The optimal conditions of the fruit were as follows: microwave power was 350 W, mass of vaporization solvent was 0 mL, microwave irradiation time was 30 s,water bath temperature was 80℃, water bath time was 60min, ethanol concentration was 60 % and solid to liquid ratio was 1:50 (g·mL^-1), under this condition, the yield of flavonoids was 39.952 mg·g^-1, it was 46.366%higher than the traditional water bath. The total flavonoids of root and stem had certain antibacterial activity against Pseudomonas aeruginosa (standard), Pseudomonas aeruginosa (drug resistance), Staphylococcus aureus (standard), Staphylococ-cus aureus (drug resistance) and Escherichia coli (standard), the MIC were 8 mg·mL^-1, 8 mg·mL^-1, 8 mg·mL^-1, 8 mg·mL^-1, 16 mg·mL^-1 respectively. The MIC of total flavonoids of fruit against the first four species of bacteria were 8 mg·mL^-1, 16 mg·mL^-1, 16 mg·mL^-1, 8 mg·mL^-1respectively. The study indicated that microwave irradiation pretreatment had significance for the extraction of total flavonoids. The antibacterial activities of total flavonoids deserve further study.
作者
管杨洋
许清遥
訾双双
罗栋
蔡远航
陈睿
GUANG Yangyang;XU Qingyao;ZI Shuangshuang;LUO Dong;CAI Yuanhang;CHEN Rui(Faculty of Ecology, Lishui University, Lishui 323000, Zhejiang)
出处
《丽水学院学报》
2019年第2期44-55,共12页
Journal of Lishui University
基金
浙江省"十三五"一流学科建设项目"生态学"(2016-2020)
关键词
地菍
生物活性
微波辐射预处理
总黄酮提取工艺
Melastoma dodecandrum Lour.
Biological activity
Microwave irradiation pretreatment
Optimization of flavonoids extraction