乳腺癌患者外周血中循环肿瘤细胞的检测及临床意义

Detection of circulating tumor cell in breast cancer and clinical significance

目的:探讨乳腺癌患者外周血中循环肿瘤细胞(circulating tumor cell,CTC)检测的临床意义。方法:应用纳米基底富集分离及免疫荧光染色方法检测CTC数目变化和CTC-Her2表达,分析CTC与临床病理特征的关系。结果:乳腺癌患者外周血中CTC的检出率明显高于乳腺良性病变(43.9%vs 0),两者比较差异有统计学意义(P<0.05)。CTC检测与乳腺癌患者年龄、肿瘤大小、组织类型、淋巴结转移及激素受体ER和PR表达无关(P>0.05)。CTC检测与脉管内查见癌栓相关(P<0.05)。组织学Her2阳性与CTC-Her2阳性具有较高的一致性。结论:CTC可能反应乳腺癌的转移能力,CTC检测可以作为判断乳腺癌预后的新指标。CTC-Her2检测可能有助于实时Her2状态的判断。

Objective:To investigate the detection of circulating tumor cell(CTC) in breast cancer and its clinical significance.Methods:Nano-basement enrichment and immunofluorescent staining were employed to detect CTC and CTC-Her2.Results:The detection rate of CTC in breast cancer was obviously higher than that in benign breast disease(43.9% vs 0).There was significant difference between breast cancer and benign breast disease(P<0.05),and it was not related to age,tumor size,histological type,lymph node metastasis and positive expression of ER and PR(P>0.05).Detection of CTC was significantly correlated with tumor emboli in blood vessels of tumor(P<0.05).Histological Her2 positive expression was obviously consistent with Her2 positive in CTC.Conclusion:CTC indicates the metastasis ability of breast cancer,and it is a new indicator to judge cancer prognosis.The detection of CTC-Her2 may help determine the real-time Her2 state.