胡椒酸乙二胺对高脂血症大鼠血脂水平和抗氧化能力的影响

Effects of pepper acid ethylenediamine on blood lipid levels and antioxidant capacity in hyperlipidemia rats

目的研究胡椒酸乙二胺(PAE)对高脂血症大鼠血脂水平和抗氧化能力的影响。方法选取SPF级雄性SD大鼠65只,测定其基础血清胆固醇(TC)和三酰甘油(TG)水平,10只作为正常对照组,给予普通饲料,55只作为造模组喂以高脂饲料,干预4周后,再次检测TC和TG,比较造模前后血脂水平,TC、TG显著升高认为造模成功。将造模成功的50只大鼠分为五组:高脂模型组(n=10);阳性药物组(辛伐他汀,10mg/kg,n=10);PAE高剂量组(10mg/kg,n=10);中剂量组(5mg/kg,n=10);低剂量组(2.5mg/kg,n=10),各组连续灌胃4周,每日1次。第4周末,采集血清,分析TC、TG、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)以及丙二醛(MDA)水平。结果与正常对照组和高脂模型组比较,PAE各剂量组均能降低TC、TG、LDL-C水平,升高HDL-C水平(P<0.05或P<0.01);与阳性药物组比较,PAE高剂量组LDL-C水平显著降低(P<0.05)。与高脂模型组比较,PAE高剂量组T-SOD、GSH-Px活性升高(P<0.05或P<0.01),阳性药物组和PAE中剂量组MDA含量显著降低(P<0.01);与阳性药物组比较,PAE高剂量组MDA含量明显升高(P<0.05)。结论PAE能降低高脂血症大鼠血脂水平,升高血清T-SOD和GSH-Px活力,降低MDA含量,提示PAE的降血脂作用可能与其改善高脂饮食大鼠氧化应激作用有关。

bjective To study the effects of pepper acid ethylenediamine(PAE)on blood lipid levels and antioxidant capacity in hyperlipidemia rats.Methods Sixty-five SPF male Sprague-Dawley rats were selected.Their basic serum cholesterol(TC)and triglyceride(TG)were determined.10 rats were selected as the normal control group and given normal diets.55 rats were fed with high-fat diet as model groups.After 4 weeks of intervention,TC and TG were detected again.Compared with the blood lipid levels before and after modeling,TC and TG were significantly increased,then the model was believed to have been built successfully.The 50 rats with successful modeling were divided into 5 groups:high-fat model group(n=10),positive drug group(Simvastatin,10 mg/kg,n=10),and high(10 mg/kg,n=10),medium(5 mg/kg,n=10)and low(2.5 mg/kg,n=10)dose of PAE groups.Each group was continuously intragastrically administered for 4 weeks,once a day.At the end of the fourth week,serum was collected for analysis of the levels of TC,TG,high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA).Results Compared with the normal control group and hyperlipidemia model group,each dose of PAE groups could reduce the levels of TC,TG LDL-C and raise HDL-C(P<0.05 or P<0.01);compared with the positive drug group,the levels of LDL-C in high dose of PAE group were decreased(P<0.05).Compared with the high fat model group,the activity of T-SOD and GSH-Px in high dose of PAE group increased(P<0.05 or P<0.01),the content of MDA in positive drug group and middle dose of PAE group was significantly decreased(P<0.01).Compared with the positive drug group,the content of MDA in high dose of PAE group inecreased(P<0.05).Conclusion PAE can reduce blood lipid levels,increase the activity of serum T-SOD,GSH-Px,and decrease MDA content in hyperlipidemia rats,suggesting that the hypolipidemic effect of PAE may be ralated to improving oxidative stress in rats with high fat diet.