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海洋低温肌氨酸氧化酶生产菌株的筛选、鉴定及酶学特性研究

Screening, identification and enzymatic characteristics of marine low-temperature sarcosine oxidase producing strain
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摘要 为获得更适应低温环境的产肌氨酸氧化酶菌株,该研究从渤海海泥中分离、筛选高产低温肌氨酸氧化酶菌株,采用形态观察、生理生化试验及分子生物学技术对其进行鉴定,并对其所产的肌氨酸氧化酶的酶学特性进行初步研究。结果表明,共分离到产肌氨酸氧化酶的菌株8株,其中菌株LYH18的肌氨酸氧化酶活力最高,为1.65 U/mL;菌株LYH18被鉴定为海泥芽孢杆菌(Bacillus oceanisediminis);肌氨酸氧化酶的最适作用温度和pH分别为25 ℃、8.0,在温度25~40 ℃、pH 7.0~10.0的范围内,酶活性较稳定,该酶属于低温酶。 In order to obtain sarcosine oxidase producing strain adapted to low-temperature environment, a high yield low-temperature sarcosine oxidase strain was isolated and screened from Bohai Sea mud, identified by morphological observation, physiological and biochemical tests and molecular biological techniques, and the enzymatic characteristics of sarcosine oxidase were preliminarily studied. The results showed that 8 sarcosine oxidase producing strains were isolated. Among them, the sarcosine oxidase activity of strain LYH18 was the highest (1.65 U/ml). The strain LYH18 was identified as Bacillus oceanisediminis. The optimum temperature and pH of the sarcosine oxidase were 25 ℃ and 8.0, respectively. The enzyme activity was stable in the range of temperature 25-40 ℃ and pH 7.0-10.0, and the enzyme belonged to low temperature enzyme.
作者 迟乃玉 刘洋 于爽 希伦 李美玉 张庆芳 CHI Naiyu;LIU Yang;YU Shuang;XI Lun;LI Meiyu;ZHANG Qingfang(College of Life Science and Biotechnology,Dalian University,Dalian 116622,China;Liaoning Marine Microbial Engineering and Technology Center,Dalian 116622,China)
出处 《中国酿造》 CAS 北大核心 2019年第6期30-34,共5页 China Brewing
基金 国家高技术研究发展计划‘863计划’(No.2018YFC0311100) 国家自然科学基金(No.31500039) 辽宁省自然科学基金项目(No.20170520167,20180550728) 大连市支持高层次人才创新创业项目(No.2017RQ155)
关键词 肌氨酸氧化酶 筛选 鉴定 酶学性质 sarcosine oxidase screening identification enzymatic property
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  • 1Suzuki K, Ogishima M, Sugiyama M, et al. Molecular cloning and expression of a Streptomyces sarcosine oxidase gene in Streptomyces lividans. Biosci Biotechnol Biochem, 1992, 56(3): 432-436.
  • 2Matsuda Y, Hoshika H, Inouye Y, et al. Purification and characterization of sarcosine oxidase of Bacillus origin. Chem Pharm Bull, 1987, 35(2): 711-717.
  • 3Ichikawa T, Sasaki H, Koike H, et al. Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129. d Struct Biol, 1997, 120(1): 109-111.
  • 4Nishiya Y, Imanaka T. Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826. J Ferment Bioeng, 1993, 75: 239-244.
  • 5Ogushi S, Nagao K, Emi S, et al. Sarcosine oxidase from Athrobacter ureafaciens: purification and some properties. Chem Pharm Bull, 1988, 36(4): 1445-1450.
  • 6Suzuki M. Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96. J Biol Chem, 1981, 89: 599-607.
  • 7Kim JM, Shimizu S, Yamada H. Crystallization and characterization of sarcosine oxidase from Alcaligenes denitrificans subsp, denitrificans. Agrie Biol Chem, 1987, 51(4): 1167-1168.
  • 8Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the Folin Phenol reagent. J Biol Chem, 1951, 193: 265-275.
  • 9Lineweaver H, Burk D. The determination of enzyme dissociation constants. J Am Chem Soc, 1934, 56: 658-666.
  • 10Toshio I, Masaru S, Taiji K, et al. New sarcosine oxidase M of specified aminoacid sequence-used for enzymatic assay ofcreatinine or creatine: JP, 5115281-A. 1993-05-14.

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