黄芪祛疣汤对尖锐湿疣患者外周血Treg/Th17细胞免疫的影响

Effects of Huangqi Quyou Decoction on Treg/Th17 Cell Immunity in Peripheral Blood of Verruca Acuminate

目的探讨黄芪祛疣汤治疗尖锐湿疣的疗效及对Treg/Th17细胞免疫的调节作用。方法纳入尖锐湿疣患者共120例作为研究对象,按随机数字表法分为对照组(60例)和治疗组(60例)。对照组采取CO2激光术治疗;涂抹5%咪喹莫特乳膏于疣体部位,隔日1次,3次/周。治疗组在对照组基础上予黄芪祛疣汤内服治疗,1剂/d,2次/d。2组疗程均2个月。比较2组患者创面愈合时间、临床疗效及复发率。检测2组外周血Treg/Th17细胞及血清白细胞介素(IL)-6、IL-17、转化生子因子(TGF)-β水平。结果治疗组创面平均愈合时间为(11.04±1.34)d,显著低于对照组为(17.15±1.92)d(P<0.05)。治疗组患者总有效率为96.67%,显著高于对照组为81.67%(P<0.05)。随访3个月,治疗组复发率为8.33%,显著低于对照组为21.67%(χ^2=4.002,P<0.05)。治疗后,治疗组患者外周血Treg,Treg/Th17显著低于对照组,Th17显著高于对照组(P<0.01)。治疗组治疗后患者血清IL-6,IL-17水平显著高于对照组,TGF-β水平显著少于对照组(P<0.01)。结论在常规干预基础上,黄芪祛疣汤治疗尖锐湿疣可促进创面愈合,提高疗效,降低复发率,调节血中Treg/Th17细胞免疫可能与其疗效有关。

Objective In order to investigate the efficacy of Huangqi Quyou decoction in treating verruca acuminate and its regulation role of regulatory T cells(Treg)/T helper cell 17(Th17)cell immunity in peripheral blood.Methods One hundred and twenty cases with verruca acuminate were selected and randomly divided into treatment group and control group with 60 in each group according to random number table method.Control group was treated with CO2 laser surgery and applied with 5%miquimod cream(one time/every two days and three times a week).On basis of control group,treatment group was treated with Huangqi Quyou decoction one dose a day and two times a day.Two groups were observed for two months.Wound healing time,clinical efficacy and recurrence rate were compared between the two groups.Peripheral blood level of Treg/Th17 and serum levels of interleukin(IL)-6,IL-17,and transforming factor(TGF)-βwere detected in both two groups.Results Wound healing time of treatment group was(11.04±1.34)d,which was obviously less than that of control group(17.15±1.92)d,(P<0.05).The efficacy of treatment group was 96.67%,which was higher than that of control group(81.67%,P<0.05).After three months of follow-up,the recurrence rate of the treatment group was 8.33%,which was significantly lower than that of the control group(21.67%;χ^2=4.002,P<0.05).Peripheral blood level of Treg and Treg/Th17 of treatment group were obviously lower,while Th17 was higher than control group after treatment(P<0.01).Serum levels of IL-6 and IL-17 of treatment group were higher after treatment,while TGF-βwas lower than that of control group(P<0.01).Conclusion On the basis of conventional intervention,Huangqi Quyou decoction in treating verruca acuminate can promote wound healing,increase the efficacy,and reduce recurrence rate.The regulation of blood Treg/Th17 immunity may be related to the curative effect.