天麻提取物提高大鼠的抗缺氧能力

Improvement of Anti-hypoxia Ability of Gastrodia elata Extract in Rats

研究天麻提取物对大鼠抗缺氧能力的作用机制。通过建立大鼠常压耐缺氧实验模型,随机分为正常组、阳性对照组、低剂量天麻提取物组和高剂量天麻提取物组,统计大鼠的体重、生存时间、生存率。检测丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、乳酸脱氢酶(LDH)、乳酸(Lactate,LA)、谷胱甘肽(GSH)、谷胱甘肽还原酶(GR)水平、热休克蛋白70(HSp70)、内皮素-1(ET-1)、一氧化氮合酶(iNOS)、缺氧诱导因子-1α(HIF-1α)相关蛋白表达。研究结果表明:高剂量天麻提取物组大鼠生存时间提高了34.01%,LDH、LA、GSH、GR水平分别为8614.24 nmol/mL、2.01 nmol/mL、965.5 mg/mL、0.30 U/mL,HSp70蛋白表达为4.14,均显著高于正常组(p<0.05);MDA、CAT水平分别为63.52 U/mL、23.24 U/mL,ET-1、iNOS、HIF-1α蛋白表达分别为1.96、2.10、0.28,均显著低于正常组(p<0.05)。说明天麻提取物能够有效的提高大鼠抗氧化能力,改善大鼠的氧化应激相关指标水平,从而提高大鼠机体的缺氧能力。

To study the mechanism of Gastrodia elata extract on anti-hypoxia ability in rats.The rats were randomly divided into normal group,positive control group,low-dose Gastrodia elata extract group and high-dose Gastrodia elata extract group.The body weight,survival time and survival rate of rats were investigated.Malondialdehyde(MDA),superoxide dismutase(SOD),total antioxidant capacity(T-AOC),catalase(CAT),lactate dehydrogenase(LDH),lactate(LA),glutathione(GSH),glutathione reductase(GR),heat shock protein 70(HSp70),endothelin-1(ET-1),nitric oxide synthase(iNOS),the expression of related proteins for hypoxia inducible factor-1alpha(HIF-1a)were detected.The results showed that,compared with the normal group,the survival time of rats in the high dose Gastrodia elata extract group increased by 34.01%,the levels of LDH(8614.24 nmol/mL),LA(2.01 nmol/mL),GSH(965.5 mg/mL),GR(0.30 U/mL)increased(p<0.05),HSp70(4.14),MDA(63.52 U/mL),CAT(23.24 U/mL),ET-1(1.96),iNOS(2.10),HIF-1a(0.28)decreased(p<0.05).These results suggested that Gastrodia elata extract can effectively improve the antioxidant capacity of rats,the level of oxidative stress in rats,and thus improve the hypoxic capacity of rats.