重症手足口病患儿外周血炎性因子、神经元特异性烯醇化酶与中枢神经特异性S100β蛋白水平变化

Changes of inflammatory factors, neuron-specific enolase and central nervous system specific protein S100β in peripheral blood of children with severe hand, foot and mouth disease

目的研究重症手足口病(HFMD)患儿外周血白细胞介素(IL)-6、IL-10、IL-17、神经元特异性烯醇化酶、中枢神经特异性蛋白S100β的变化。方法以60例手足口病患儿为研究对象,其中轻症患儿30例(轻症组)和重症30例(重症组),另选取30例健康儿童为对照组;采用双抗体夹心酶联免疫吸附实验检测各组患儿外周血IL-6、IL-10、IL-17、NSE和S100β水平。结果与对照组、轻症患儿相比,重症手足口病患儿外周血IL-6、IL-10、IL-17水平均显著升高,差异有统计学意义(重症组vs.对照组:t=9.83、8.56、7.85,P=0.043、0.019、0.011;重症组vs.轻症组:t=5.84、4.95、6.59,P=0.023、0.032、0.024)。与对照组患儿相比,轻症患儿外周血IL-6、IL-10、IL-17水平稍有升高,但差异无统计学意义(t=0.53、1.03、0.38,P=0.292、0.445、0.362)。与对照组和轻症组相比,重症组患儿外周血NSE和S100β的水平显著升高,差异有统计学意义(重症组vs.对照组:t=11.39、9.25,P=0.018、0.016;重症组vs.轻症组:t=5.44、6.3,P=0.031、0.028)。与对照组相比,轻症患儿外周血NSE、S100β均有升高,但无统计学意义(t=1.38、1.89,P=0.098、0.142)。结论手足口病患儿外周血IL-6、IL-10、IL-17、NSE和S100β水平升高,可提示病情严重程度;临床监测以上指标变化,可早期发现重症患者,尽早治疗。

Objective To investigate the changes of interleukin(IL)-6,IL-10,IL17,neuron-specific enolase and central nervous specific protein S100βof peripheral blood in children with hand,foot and mouth disease(HFMD).Methods Total of 60 children with HFMD were collected,including 30 children with mild symptoms(mild group)and 30 children with severe symptoms(severe group),while 30 healthy children were selected as control group.The expression of IL-6,IL-10,IL-17,NSE and S100βin peripheral blood of children in each group were detected by double antibody sandwich enzyme-linked immunosorbent assay(ELISA).Results The levels of IL-6,IL-10 and IL-17 in peripheral blood of children in severe group were significantly higher than those of the control group and mild group,all with significant differences(severe group vs.control group:t=9.83,8.56,7.85;P=0.043,0.019,0.011.severe group vs.mild group:t=5.84,4.95,6.59;P=0.023,0.032,0.024).Compared with the control group,the levels of IL-6,IL-10 and IL-17 in peripheral blood of children in mild group were slightly higher,but there were no significant differences(t=0.53,1.03,0.38;P=0.292,0.445,0.362).Compared with the control group and mild group,the levels of NSE and S100βin peripheral blood of children in severe group were significantly higher,with significant differences(severe group vs.control group:t=11.39,9.25;P=0.018,0.016.severe group vs.mild group:t=5.44,6.30;P=0.031,0.028).Compared with the control group,the levels of NSE and S100βin peripheral blood of children in mild group were higher,but with no significant differences(t=1.38,1.89;P=0.098,0.142).Conclusions The levels of IL-6,IL-10,IL-17,NSE and S100βin peripheral blood of children with HFMD were increased,which could indicate the severity of the disease,and the changes of the above indexes could be detected in the early stage of severe patients and treated as soon as possible.