HPLC法同时测定良姜胃疡胶囊中7种成分

Simultaneous determination of seven constituents in Liangjiang Weiyang Capsules by HPLC

目的建立HPLC法同时测定良姜胃疡胶囊(吴茱萸、高良姜、藿香等)中东莨菪苷、异嗪皮啶、东莨菪内酯、吴茱萸碱、吴茱萸次碱、吴茱萸新碱、去氢吴茱萸碱的含有量。方法该药物70%甲醇提取液的分析采用Diamonsil C18色谱柱(150 mm×4.6 mm,5μm);流动相甲醇-0.1%甲酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长267、334 nm。结果7种成分在各自范围内线性关系良好(r≥0.999 7),平均加样回收率98.84%~102.63%,RSD 0.69%~2.00%。结论该方法简便准确,重复性好,可用于良姜胃疡胶囊的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of scopolin,iso- fraxidin,scopoletin, evodiamine, rutecarpine, evocarpine, and dehydroevodiamine in Liangjiang Weiyang Capsules ( Evodia rutaecarpa,Alpinia officinarum,Agastache rugosa,etc.). METHODS The analysis of 70% methanol extract of this drug was performed on a 30 ℃ themostatic Diamonsil C18 column ( 150 mm × 4. 6 mm, 5 μm),with the mobile phase comprising of methanol-0. 1% formic acid flowing at 1. 0 mL /min in a gradient elu- tion manner,and the detection wavelengths were set at 267,334 nm. RESULTS Seven constituents showed good linear relationships within their own ranges ( r≥0. 999 7),whose average recoveries were 98. 84%-102. 63% with the RSDs of 0. 69%-2. 00%. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Liangjiang Weiyang Capsules.