HER-2与VEGF-C在胃癌组织中的表达及其相关性研究

The expression of HER-2 and vascular endothelial growth factor-C in gastric cancer and their correlation

目的探讨HER-2与VEGF-C在胃癌组织中的表达情况及其与胃癌不同临床病理特征之间的相关性.方法 61例行胃癌D2根治术患者手术标本.应用免疫组化技术分别检测胃癌组织中HER-2及VEGF-C的表达情况,比较胃癌不同临床病理特征间表达差异,并收集患者性别、年龄、肿瘤直径大小、浸润程度、分型、分期、组织学分级、淋巴结转移情况及肿瘤所在部位等信息.结果 HER-2和VEGF-C阳性表达率分别为11.5%和49.2%.二者无明显相关性(P>0.05).HER-2表达阳性者,性别、年龄、肿瘤直径、肿瘤位置、浸润深度、各分型、各分期、分化程度及淋巴转移情况之间差异无明显统计学意义(P>0.05).VEGF表达阳性者,性别、年龄、肿瘤直径、肿瘤位置、浸润深度、各分型、分化程度之间差异无明显统计学意义(P>0.05).VEGF表达阳性者,0期0/0例,Ⅰ期10/19例,Ⅱ期4/16例,Ⅲ期17/26例,Ⅳ期0/0例.阳性表达率分别为:0,52.6%,25.0%,65.4%,0,三者差异有统计学意义(P<0.05).Ⅱ期、Ⅲ期比较,差异有统计学意义(P<0.017).Ⅲ期癌组织中VEGF-C的阳性表达率较Ⅱ期癌组织中高.其他组间比较差异无统计学意义(P>0.017).VEGF表达阳性者,有淋巴结转移30/50例,无淋巴结转移3/11例.阳性表达率分别为:60.0%,27.3%,两者差异有统计学意义(P<0.05).结论(1)HER-2与VEGF-C在胃癌组织中过表达.(2)HER-2阳性表达率与胃癌患者性别、年龄、肿瘤大小、浸润深度、肿瘤分期、组织分化程度、分型及淋巴结转移等临床特征间无明显相关性.(3)VEGF-C阳性表达率与胃癌患者性别、年龄、肿瘤大小、浸润深度、肿瘤组织分化程度、分型等临床特征之间无明显相关性.(4)VEGF-C阳性表达率与胃癌分期及淋巴结转移之间存在相关性:在有淋巴结转移的癌组织中VEGF-C阳性表达率高于无淋巴结转移癌组织;晚期胃癌VEGF-C阳性表达率比早期高.(5)HER-2与VEGF-C二者表达之间相关性不显著.

Objective To research the expression of HER-2 and vascular endothelial growth factor-C(VEGF-C) in gastric cancer tissue and to study the correlation among different pathological features,clinical characteristics. Methods According to the limited criteria,specimens from patients underwent radical resection of gastric cancer in Yi Jishan Hospital of Wannan Medical College. Characteristic of patients included gender,age, tumor size,degree of infiltration diameter,classification and stage,histological grade,lymph node metastasis and tumor's location were collected. The expression of HER-2 and VEGF-C were tested by immunohistochemical,and then the expression difference in different clinical factors of gastric cancer were compared. Results Of 61 cases with gastric cancer tissues,HER-2 was detected in 7 cases(11.5%),and VEGF-C was detected in cases (49.2%)in this study. Our study founded that there was no significant correlation between positive expression of A and B. For the positive expression of HER-2,there was no significant difference in gender,age,tumor size,degree of infiltration diameter,classification and stage,histological grade, lymph node metastasis and tumor's location(P>0.05). In this study,For the positive expression of VEGF-C,there was no significant difference in gender,age,tumor size,degree of infiltration diameter,histological grade,and tumor's location(P>0.05). There was obviously difference in positive rate of VEGF-C in the gastric cancer tissue in lymphatic metastasis and clinical stages(P<0.05).Positive rate of VEGF-C with lymphatic metastasis or not were respectively 60.0%(30/60)and27.3%(3/11).The positive expression of VEGF-C was significantly correlated with TNM stage(gradeⅠ,Ⅱ,Ⅲ),the rates of expression were respectively 52.6%,25.0%,65.4%. We noted that there was a significant difference between gradeⅡand grade Ⅲ(P<0.05),but not for other combinations. Conclusion (1)HER-2 and VEGF-C are over-expressed in gastric cancer tissues.(2)There is no obviously correlation between the positive expression rate of HER-2 and patients' gender,age,tumor size,invasion depth,tumor stage,tissue differentiation degree,classification and lymphatic metastasis and clinical features.(3)There is no obviously correlation between the positive expression rate of and patients' gender,age,tumor size,invasion depth,the degree of tumor differentiation,classification.(4)Positive expression rate of VEGF-C is associated with the gastric cancer stages and lymphatic metastasis:compared with carcinoma tissues,not lymphatic metastasis in cancerous tissue. The positive expression rate of VEGF-C in tissues with lymphatic metastasis is higher.(5)There is no significant correlation between the expression of HER-2 and VEGF-C.