趋化因子CCL5和基质金属蛋白酶9表达水平与非小细胞肺癌术后复发的相关性

Correlation between Serum Levels of Chemokine CCL5 and Metalloproteinase MMP-9 and Postoperative Recurrence of Human Non-small Cell Lung Cancer

【目的】分析趋化因子CCL5和基质金属蛋白酶9(MMP-9)表达水平与非小细胞肺癌(NSCLC)术后复发的相关性。【方法】收集本院诊治的103例NSCLC患者(观察组)和同期本院健康志愿者20例(对照组),检测NSCLC患者术后3个月和健康对照组血清CCL5及MMP-9水平,以术后3个月CCL5和MMP-9中位数水平将患者分为CCL5、MMP-9高水平组与CCL5、MMP-9低水平组,随访2年,比较CCL5、MMP-9高水平组与低水平组复发率,并分析CCL5及MMP-9与患者术后复发的相关性。【结果】CCL5高水平组复发率为53.12%(17/32),明显高于CCL5低水平组的29.58%(21/71),其差异有统计学意义(P<0.05);MMP-9高水平组复发率为46.67%(14/30),高于MMP-9低水平组的32.88%(24/73),差异有统计学意义(P<0.05)。相关性分析显示:术后3个月的血清CCL5及MMP-9水平与肿瘤复发明显相关(P<0.05),其他指标与术后复发无相关性(P>0.05)。【结论】血清趋化因子CCL5及MMP-9水平的异常升高可能在NSCLC发生、发展过程中起重要作用,检测NSCLC患者术后血清CCL5及MMP-9水平变化有助于早期监测肿瘤复发。

【Objective】To study the expression of chemokine CCL5 and MMP-9 in serum of human non-small cell lung cancer and their relationship with the postoperative recurrence.【Methods】A prospective cohort study was conducted to investigate the serum levels of CCL5 and MMP-9 in 103 patients with NSCLC.Serum samples of all patients were collected at 3 months after operation.Serum samples of 20 healthy subjects were collected to detect the levels of CCL5 and MMP-9 as well.The patients were divided into the high level group and low level group of CCL5 and MMP-9 based on the median level of serum CCL5 and MMP-9 at 3 months postoperation.After two years of follow-up,the difference in recurrence rates between high-level group and low-level group was observed,and the correlation between level of CCL5 and MMP-9 and postoperative recurrence rate was analyzed.【Results】The recurrence rate of CCL5 high-level group was 53.12%(17/32),which was significantly higher than that of CCL5 low-level group (29.58%,21/71).The difference was statistically significant (P<0.05).The recurrence rate of MMP-9 high-level group was 46.67%(14/30),which was higher than that of MMP-9 low level group (32.88%,24/73).The difference was statistically significant (P<0.05).The Cox regression analysis showed that the high levels of serum CCL5 and MMP-9 after three months of operation were correlated to the recurrence of NSCLC (P<0.05).No other indexes were not associated with postoperative recurrence (P>0.05).【Conclusion】The abnormal elevated levels of serum chemokine CCL5 and MMP-9 may play an important role in the occurrence and development of NSCLC.Detecting the changes of serum CCL5 and MMP-9 levels after operation may be helpful for early warning of tumor recurrence.