游离脂肪酸受体FFAR4/GPR120在乳腺癌组织中的表达及其临床意义

Expression and clinical significance of free fatty acid receptor FFAR4/GPR120 in breast cancer

目的观察GPR120蛋白在乳腺癌患者癌组织与癌旁正常组织中的表达,并分析其表达与临床病理特征之间的关系。方法收集2016年10月—2017年10月于宁夏医科大学总医院肿瘤医院肿瘤外一科行手术获取的乳腺癌石蜡标本125份及癌旁正常组织标本63份,采用免疫组织化学染色方法检测GPR120蛋白在2种组织中的表达情况,结合临床病理资料进行统计学分析。结果GPR120在乳腺癌组织中呈高表达,阳性率为90.4%,明显高于癌旁正常组织31.7%(χ^2/P=69.629/0.000);GPR120蛋白在乳腺癌不同的分子分型中表达差异均无统计学意义(χ^2/P=5.859/0.119);GPR120蛋白表达在浸润性小叶癌、浸润性导管癌及其他类型癌中的阳性表达率依次升高,分别为75.0%、93.7%和100%,差异具有统计学意义(χ^2/P=8.186/0.017);GPR120蛋白表达与Ki-67表达呈正相关,与ER的表达呈负相关(r/P=0.220/0.014、-0.232/0.009)。结论GPR120蛋白在人乳腺癌组织中呈高表达且与Ki-67及ER的表达相关,可能在乳腺癌的发展中起促癌作用。

Objective To observe the expression of GPR120 protein in breast cancer tissues and adjacent normal tissues,and to analyze the relationship between its expression and clinicopathological characteristics.Methods From October 2016 to October 2017,125 paraffin specimens of breast cancer and 63 normal tissue specimens were collected from the Department of Surgery,Oncology Hospital,General Hospital of Ningxia Medical University.Immunohistochemical staining was used to detect the expression of GPR120 protein in two tissues.Statistical analysis was performed with clinical and pathological data.Results The positive rate of GPR120 in breast cancer tissue was 90.4%,which was significantly higher than that in normal adjacent tissues(31.7%)(χ^2/P=69.629/0.000).There was no significant difference in the expression of GPR120 protein in different molecular types of breast cancer(χ^2/P=5.859/0.119);the positive expression rate of GPR120 protein in invasive lobular carcinoma,invasive ductal carcinoma and other types of cancer increased in turn,which was 75.0%,93.7%,and 100%,respectively.The difference was statistically significant(χ^2/P=8.186/0.01).GPR120 expression was positively correlated with Ki 67 expression and negatively correlated with ER expression(r/P=0.220/0.014,-0.232/0.009).Conclusion GPR120 protein is highly expressed in human breast cancer tissues and is associated with the expression of Ki 67 and ER,which may play a role in promoting the development of breast cancer.