安徽“粉性葛”与“柴性葛”中6种异黄酮类化合物含量比较

Comparison of the Content of Six Isoflavones in the Mealy-like Roots and the Wooden-like Roots of Pueraria lobata from Anhui Province,China

目的采用高效液相色谱法同时测定安徽省“粉性葛”与“柴性葛”中6种异黄酮化合物含量。方法采用Agilent Zorbax XDB-C18色谱柱(250mm×4.6mm,3.5μm),流动相为甲醇(A)-0.2%醋酸水(B),0.8mL/min梯度洗脱,检测波长250nm,柱温30℃。结果26批葛根样品中6种异黄酮化合物在48min内完全分离且线性关系良好(r>0.999),3′-羟基葛根素、葛根素、3′-甲氧基葛根素、葛根素芹菜糖苷、大豆苷、大豆苷元的平均回收率分别为98.6%、103.4%、98.5%、102.1%、105.9%、100.7%(n=6)。聚类分析和主成分分析结果表明,“粉性葛”与“柴性葛”分别聚为两类。结论“粉性葛”与“柴性葛”中异黄酮类化合物的含量有差异,其中,“粉性葛”中葛根素含量较“柴性葛”偏低。

Objective To simultaneously determine the content of six isoflavones in the mealy-like roots and the wooden-like roots of Pueraria lobata from Anhui Province,China using high-performance liquid chromatography(HPLC).Methods HPLC was performed on an Agilent Zorbax XDB-C 18 column(250 mm×4.6 mm,3.5μm)with a mobile phase of methanol(A)-0.2%acetic acid(B)for gradient elution,at a flow rate of 0.8 mL/min,a detection wavelength of 250 nm,and a column temperature of 30℃.Results In the 26 batches of Pueraria lobata roots,the six isoflavones were completely separated within 48 minutes and had a good linear relationship(r>0.999).The average recovery rates of 3′-hydroxy puerarin,puerarin,3′-methoxy puerarin,puerarin celery glycoside,daidzin,and daidzein in Pueraria lobata roots were 98.6%,103.4%,98.5%,102.1%,105.9%,and 100.7%,respectively(n=6).The results of the cluster analysis and the principal component analysis showed that the mealy-like roots and the wooden-like roots were divided into two categories.Conclusion There is a great difference in the content of isoflavones between the mealy-like roots and the wooden-like roots of Pueraria lobate,and the mealy-like roots have a lower content of puerarin than the wooden-like roots.