摘要
导出了共焦显微镜中不同荧光波长情况下的荧光功率传输函数、三维脉冲响应函数 (3D PSF)和三维光学传递函数 (3D OTF)。结果表明 ,不同的荧光波长对共焦显微镜的空间截止频率、分辨率、光学传递函数存在明显的影响。当激发波长与荧光波长的比值下降到一定程度时 。
Fluorescence power transfer function, three-dimensional point spread function (3D-PSF) and three-dimensional optical transfer function (3D-OTF) for the various fluorescent wavelength are calculated. The results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut-off frequency, resolution and 3D-OTF. There is a missing-cone in the 3-D space of OTF when the ratio of excitation wavelength to fluorescent wavelength decreases.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2002年第10期1219-1223,共5页
Acta Optica Sinica
基金
广东省自然科学基金 (980 0 4 6 )资助课题
关键词
荧光波长
共焦显微镜
成像特性
失锥现象
Fluorescence
Imaging systems
Optical transfer function
Three dimensional