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荧光波长对共焦显微镜成像特性的影响 被引量:8

Influence of Fluorescent Wavelength on Imaging Property of Confocal Microscopy
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摘要 导出了共焦显微镜中不同荧光波长情况下的荧光功率传输函数、三维脉冲响应函数 (3D PSF)和三维光学传递函数 (3D OTF)。结果表明 ,不同的荧光波长对共焦显微镜的空间截止频率、分辨率、光学传递函数存在明显的影响。当激发波长与荧光波长的比值下降到一定程度时 。 Fluorescence power transfer function, three-dimensional point spread function (3D-PSF) and three-dimensional optical transfer function (3D-OTF) for the various fluorescent wavelength are calculated. The results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut-off frequency, resolution and 3D-OTF. There is a missing-cone in the 3-D space of OTF when the ratio of excitation wavelength to fluorescent wavelength decreases.
出处 《光学学报》 EI CAS CSCD 北大核心 2002年第10期1219-1223,共5页 Acta Optica Sinica
基金 广东省自然科学基金 (980 0 4 6 )资助课题
关键词 荧光波长 共焦显微镜 成像特性 失锥现象 Fluorescence Imaging systems Optical transfer function Three dimensional
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共引文献42

同被引文献75

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