用基于PCR的分子标记分析甘蓝型油菜抗感菌核病品系间的遗传距离

Genetic distance estimation for disease resistant and susceptible accessions of Brassica napus using PCR based markers

用离体叶接种法鉴定了甘蓝型油菜选系对菌核病的抗感性，结果表明绝大多数抗性品系与感病品种间的差异达到显著或极显著水平。以两种基于ＰＣＲ的标记估计所选１０ 份材料间的遗传距离。１９ 个随机引物扩增出了２６ 条重复性很好的ＲＡＰＤ 标记，１ 对依据植物Ｒ基因的ＮＢＳ区段设计的简并引物扩增出了３ 条多态性的带。依据２９ 个标记进行聚类分析，结果表明抗、感性材料在遗传距离为０．１２０６ 处被明显地区分开。５份抗性材料中，宁ＲＳ—１ 与中油８２１、中Ｒ８８８

Detached leaves from ten selected cultivars or lines of Brassica napus were inoculated to verify their response to Sclerotinia sclerotiorum based on the lesion diameter. Statistical analysis showed that the differences among the accessions were very significant. Most of resistant lines differed significantly or very significantly from four susceptible lines. Two types of PCR markers, RAPD and STS, were performed to estimate the genetic distances among the ten selected lines. Twenty six distinguished polymorphic bands were repeatedly detected using 19 random primers. On the other hand, three polymorphic bands were observed with one pair of degenerate primers that were designed according to the conservative NBS regions of R genes in Arabidopsis and other plants. All of the data of polymorphic bands were mixed to estimate the genetic diversity of the samples. Clust (UPGMA) results showed that ten accessions of B.napus were classed into two groups, resistant and susceptible group, at the genetic distance point 0.1206. As to the resistant group, Ning RS—1 was separated from other lines.