高糖诱导的人晶状体上皮细胞葡萄糖关键代谢酶的表达

High glucose induced glucose-key metabolic enzyme expression in lens epithelial cells

目的:探讨葡萄糖关键代谢酶在高糖诱导的人晶状体上皮(HLEB3)细胞中的表达情况。方法:将体外培养的HLEB3细胞分为正常对照组(采用DMEM培养液培养,含葡萄糖5mmol/L)、氧化应激组(采用含H2O2 200μmol/L的DMEM培养液培养)、高糖诱导组(采用含葡萄糖30mmol/L的培养液培养),培养24h后检测细胞凋亡情况和细胞活力及6种葡萄糖关键代谢酶(6-磷酸果糖激酶-1、丙酮酸激酶、己糖激酶、柠檬酸合成酶、α-酮戊二酸脱氢酶、6-磷酸葡萄糖脱氢酶)的mRNA表达情况。结果:高糖诱导HLEB3细胞凋亡,高糖诱导组(63.43%±3.40%)细胞活力低于正常对照组(100.00%±0.00%)和氧化应激组(91.90%±5.11%),且高糖诱导组细胞6种葡萄糖代谢关键酶的mRNA表达水平均低于正常对照组和氧化应激组(均P<0.05)。结论:高糖可以诱导HLEB3细胞葡萄糖关键代谢酶的表达水平降低,诱导细胞凋亡,影响细胞活性。

AIM:To investigate the expression of glucose key metabolic enzymes in human lens epithelial cells(HLEB3)induced by high glucose.METHODS:HLEB3 cells cultured in vitro were divided into normal control group(DMEM medium containing 5mmol/L of glucose),oxidative stress group(DMEM medium containing 5mmol/L of glucose and 200μmol/L of hydrogen peroxide),high glucose induction group(DMEM medium containing 30mmol/L of glucose).Apoptosis and cells were detected 24h after culture.Activity and expression of six key glucose metabolic enzymes(fructose-6-phosphate kinase-1,pyruvate kinase,hexokinase,citrate synthase,α-ketoglutarate dehydrogenase and 6-phosphate glucose dehydrogenase)were studied.RESULTS:Apoptosis of HLEB3 cells was induced by high glucose.The cell viability of high glucose-induced group(63.43%±3.40%)was lower than that of normal control group(100.00%±0.00%)and oxidative stress group(91.90%±5.11%),and the expression levels of six key enzymes of glucose metabolism in high glucose-induced group were lower than that of normal control group and oxidative stress group(all P<0.05).CONCLUSION:High glucose can induce the expression of glucose-key metabolizing enzymes in HLEB3 cells to decrease,induce apoptosis and affect cell activity.