新柏氏液基薄层细胞学检验联合HPV-DNA检测在诊断宫颈病变中的应用

Application of thinprep cytologic test combined with HPV-DNA detection in diagnosis of cervical lesions

目的探讨新柏氏液基薄层细胞学检验(TCT)联合HPV-DNA检测在诊断宫颈病变中的应用效果。方法选取2017年6月~2018年5月我院妇产科收治的疑为宫颈病变的66例患者作为研究对象,对其分别实施TCT检验和HPV-DNA检测,以宫颈活检结果为金标准,分析单独TCT检验、HPV-DNA检测以及联合检验在宫颈病变中的诊断敏感性、特异性和准确性。结果TCT联合HPV-DNA检测的准确性(95.45%)、敏感性(95.83%)和特异性(94.44%)均高于单独TCT检验(63.64%、68.75%、50.00%)和单独HPV-DNA检测(68.18%、72.92%、55.56%),差异有统计学意义(P<0.05)。单独TCT检验和单独HPV-DNA检测的敏感性、特异性和准确性比较,差异无统计学意义(P>0.05)。结论在早期宫颈病变患者的诊断中,采用TCT检验联合HPV-DNA检测有利于在早期诊断患者的病变性质,其诊断准确性较高,能够为临床治疗提供可靠的参考依据,在临床上值得进一步使用。

Objective To investigate the application effect of thinprep cytologic test(TCT)combined with HPV-DNA detection in the diagnosis of cervical lesions.Methods Sixty-six patients with suspected cervical lesions enrolled in the Department of Obstetrics and Gynecology of our hospital from June 2017 to May 2018 were selected as the subjects.The TCT test and HPV-DNA test were performed respectively.The results of cervical biopsy were used as the gold standard.The sensitivity,specificity,and accuracy of TCT alone,HPV-DNA alone,and joint detections in cervical lesions were analyzed.Results The accuracy,sensitivity and specificity of detections in combination were 95.45%,95.83%,and 94.44%,higher than those of TCT alone accuracy,sensitivity and specificity(63.64%,68.75%,and 50.00%)and HPV-DNA alone(68.18%,72.92%,and 55.56%)in turn,with significant differences(P<0.05).There was no significant difference in sensitivity,specificity or sensitivity of TCT alone and HPV-DNA alone(P>0.05).Conclusion In the diagnosis of patients with early cervical lesions,the combined use of TCT and HPV-DNA tests is beneficial to the early diagnosis of its nature.The diagnostic accuracy is high,and can provide a reliable reference for clinical treatment.It is worth further in clinical practice.