细胞自噬研究的策略、方法及其在中医药干预非酒精性脂肪性肝病机制中的研究现状与前景

Strategies and methods of cell autophagy research and research status and prospects in mechanisms of tcm intervention on non- alcoholic fatty liver disease

探讨细胞自噬研究的策略、误区、技术方法及优选方案,以及自噬在非酒精性脂肪性肝病(NAFLD)中的调控机制,并阐述中医药干预NAFLD的自噬机制研究现状。认为自噬在NAFLD发病(以初期为主)及治疗过程中可发挥肝细胞保护作用,并在一定程度上延缓病情的发展,自噬的这种保护效应也可能是中医“已病防变”治未病思想在中药微观效应机制中的体现。自噬目前在中医药干预疾病的机制研究领域方兴未艾,具有广阔的研究前景,但也存在有待突破的瓶颈。或可在中药标准化建设取得阶段性成果的基础上,通过开发新的中医药体外研究技术,结合高通量生物信息采集和前沿生物分析技术,取得自噬研究在中医药领域的新进展。

The strategies, misconceptions, technical methods and preferred protocols of cell autophagy research, as well as the regulatory mechanism of autophagy in non-alcoholic fatty liver disease(NAFLD) are discussed. At the same time, the current status of research on autophagy mechanism in TCM treatment of NAFLD is discussed. Autophagy is believed to protect hepatocytes in pathogenesis(mainly the early stage) and treatment of NAFLD, and to some extent delay the development of the disease. The protective effect of autophagy may also be reflected in the micro-effect mechanism of traditional Chinese medicine in the treatment of potential diseases by "preventing development and transmission of diseases". Autophagy is currently in the ascendant in the research of mechanism of Chinese medicine intervention in diseases and has broad research prospects. However, there are still research bottlenecks. Based on the staged achievements in the standardization of traditional Chinese medicine, it is possible to speed up the autophagy research in traditional Chinese medicine by developing new in-vitro techniques, combined with high-throughput bioinformation collection and cutting-edge bioanalytical techniques.