女贞子提取物中齐墩果酸含量及抗肿瘤活性研究

Study on the Content of Oleanolic Acid and Antitumor Activity from Fructus Ligustri Lucidi Extracts

比较不同提取工艺对女贞子中齐墩果酸含量的影响及研究女贞子提取物的体外抗肿瘤活性。该试验以女贞子为原料,以齐墩果酸为对照品,利用紫外分光光度法对提取的齐墩果酸含量进行精密测定,利用MTT法检测提取物对细胞增殖的影响。结果显示,4种不同提取方法中的齐墩果酸含量由高到低依次为回流提取法(13.13±0.03)mg/g、索氏提取法(12.19±0.03)mg/g、超声提取法(11.56±0.02)mg/g、冷浸法(11.16±0.02)mg/g;女贞子提取物对人结肠癌细胞HCT116、人肝癌细胞HEPG2的IC50值分别为22.08、13.47 g/mL,齐墩果酸标准品对人结肠癌细胞HCT116、人肝癌细胞HEPG2的IC50值分别为41.45、45.90 g/mL。以上结果表明:乙醇回流提取效果最佳,简单易行;女贞子提取物对人结肠癌、人肝癌细胞均有抑制作用且对人肝癌细胞的抑制作用更明显。

To compare the effects of different extraction process on the oleanolic acid content in Fructus Ligustri Lucidi and the antitumor activity of Fructus Ligustri Lucidi extract in vitro was studied.The Fructus Ligustri Lucidi was used as the raw material,and the oleanolic acid was used as the reference substance.The content of oleanolic acid was determined by ultraviolet-visible spectrophotometry,and MTT was used to detect the effect of extract on cell proliferation.The results obtained that,the four different extraction methods of oleanolic acid content in order from high to low was ethanol refluxing extraction method(13.13±0.03)mg/g,soxhlet extraction method(12.19±0.03)mg/g,ultrasonic extraction method(11.56±0.02)mg/g,cold maceration method(11.16±0.02)mg/g;The IC50 values of extracts of Fructus Ligustri Lucidi on human colon cancer cells and human liver cancer cells were 22.08,13.47 g/mL,The IC50 values of the standard oleanolic acid for human colon cancer cells and human liver cancer cells respectively were 41.45,45.90 g/mL.The results showed that the ethanol refluxing extraction had the best effect and was simple and easy to carry out;the extract of Fructus Ligustri Lucidi had inhibitory effects on human colon cancer and human liver cancer cells,and inhibited human liver cancer cells was more obvious.