外源5-氨基乙酰丙酸对盐胁迫下花椰菜幼苗生理特性的影响

Effects of Exogenous 5-Aminolevulinic Acid on Physiological Characteristics of Cauliflower Seedlings under Salt Stress

以花椰菜‘津品70’为试验材料,探究150 mmol·L^-1NaCl胁迫下不同浓度外源5-氨基乙酰丙酸(5-ALA)(0,1,10,25,50和100 mg·L^-1,分别记作CK2、T1、T2、T3、T4、T5)对花椰菜幼苗生理特性的影响,以正常培养的花椰菜幼苗为CK1,分别测定各处理超氧化物歧化酶(SOD)和过氧化物酶(POD)活性及丙二醛(MDA)、可溶性蛋白和可溶性糖含量。结果表明:在150 mmol·L^-1NaCl胁迫下,T2和T5处理组花椰菜幼苗SOD活性与CK1差异不显著(P>0.05),三者显著高于CK2(盐对照)和其他5-ALA处理组(P<0.05);T1和T2处理组POD活性与CK1差异不显著(P>0.05),三者显著高于CK2和T4、T5处理组(P<0.05);T1、T2和T4处理组可溶性蛋白含量显著高于CK1和CK2(P<0.05);T3处理组可溶性糖含量显著高于CK1、CK2和其他5-ALA处理组(P<0.05);T2和T3处理组MDA含量显著低于CK1和CK2(P<0.05)。隶属函数综合评价表明,150 mmol·L^-1 NaCl胁迫下,T2处理组(10 mg·L^-15-ALA)对缓解花椰菜幼苗盐害效果最佳。

In this paper,cauliflower’Jinpin 70’was used as the experimental material to explore the effects of different concentrations of 5-aminolevulinic acid(5-ALA)(0,1,10,25,50 and 100 mg·L^-1,recording as CK2,T1,T2,T3,T4,T5)on physiological characteristics of cauliflower seedlings under 150 mmol·L^-1 NaCl stress,the normal cauliflower seedling was recorded as CK1.The activities of superoxide dismutase(SOD)and peroxidase(POD)and the contents of malondialdehyde(MDA),soluble protein and soluble sugar were measured.The results showed that under 150 mmol·L^-1 NaCl stress,the SOD activities of T2 and T5 treatments had no significant difference with CK1(P>0.05),however,the SOD activities of T2,T5 and CK1 were significantly higher than those of CK2 and other 5-ALA treatments(P<0.05);the POD activities of T1 and T2 treatments had no significant difference with CK1(P>0.05),however,the POD activities of T1,T2 and CK1 were significantly higher than those of CK2,T4 and T5 treatments(P<0.05);the soluble protein contents of T1,T2 and T4 treatments were significantly higher than those of CK1 and CK2(P<0.05);the soluble sugar content of T3 treatment was significantly higher than that of CK1,CK2 and other 5-ALA treatments(P<0.05);the MDA contents of T2 and T3 treatments were significantly lower than those of CK1 and CK2(P<0.05).The evaluation by membership function analysis indicated that T2 treatment(10 mg·L^-15-ALA)had the best effects on alleviating salt stress in cauliflower seedlings under 150 mmol·L^-1 NaCl stress.