扬麦系列品种(系)重要性状功能基因的KASP检测

Kompetitive allele specific PCR(KASP) assays for functional genes of important trait in Yangmai series wheat cultivars(lines)

为了解扬麦系列品种(系)重要性状功能基因组成,利用高通量KASP标记技术对30份扬麦系列品种(系)的株高、光周期、抗病虫、抗穗发芽、抗旱、籽粒及品质性状等相关功能基因进行检测。结果表明:76.7%的供试品种(系)含有矮秆基因Rht-B1b;所有品种(系)携带光周期不敏感基因Ppd-A1a和Ppd-D1a,均聚合多个高千粒质量基因TaSus1-7A、TaSus2-2A、TaGS-D1和TaGW2-6B等;扬麦系列品种(系)主要含有TaPHS1、Vp-B1和TaSdr-B13个抗穗发芽基因,频率分别为73.3%、90.0%和73.3%;抗旱基因CWI-4A、CWI-5D、TaMoc-A1、TaSST-A2、TaSST-D1、Dreb-B1和1-feh-w3的频率分别为66.7%、100.0%、13.3%、100.0%、93.3%、20.0%和90.0%;抗赤霉病基因Fhb1在扬麦系列品种(系)中的比例不高,仅为16.7%;70%扬麦系列品种(系)含有抗叶锈病基因Lr14a;抗禾谷孢囊线虫病基因Cre8、抗眼斑病基因Pch1、抗秆锈病基因(Sr2和Sr36)、抗叶锈病基因(Lr21、Lr34、Lr47和Lr67)、抗褐斑病基因Tsn1在供试材料中均未发现,扬麦系列品种(系)多为弱筋小麦,50%的扬麦系列品种(系)Glu-A1位点为1,86.7%的Glu-D1位点为2+12,除扬麦3号外,供试品种Glu-B3位点都为c。本研究明确了扬麦系列品种(系)部分重要性状功能基因的组成,为扬麦系列品种(系)在生产和育种上应用提供了理论依据。

In order to understand the composition of functional genes of the important traits in 30 Yangmai series wheat cultivars(lines),high-throughput kompetitive allele specific PCR(KASP)assay was used to detect some traits-associated genes related to plant height,photoperiodism,disease and pest resistance,pre-harvest sprouting resistance,drought resistance,kernel and quality traits.The results showed that 76.7%of the tested cultivars(lines)contained Rht-B1b.Photoperiod insensitivity genes Ppd-A1a and Ppd-D1a were detected in all the tested cultivars(lines).Grain weight related genes TaSus1-7A,TaSus2-2A,TaGS-D1 and TaGW2-6B were accumulated into Yangmai series wheat cultivars(lines).There were three pre-harvest sprouting resistance genes TaPHS1,Vp-B1 and TaSdr-B1 in Yangmai series wheat cultivars(lines),and the frequencies were 73.3%,90.0%and 73.3%,respectively.The frequencies of drought resistance genes CWI-4A,CWI-5D,TaMoc-A1,TaSST-A2,TaSST-D1,Dreb-B1 and 1-feh-w3 were 66.7%,100.0%,13.3%,100.0%,93.3%,20.0%and 90.0%,respectively.The fusarium head blight resistance gene Fhb1 could be detected only in five cultivars.On disease resistance,70%cultivars(lines)contained leaf rust resistance gene Lr14a.The cereal cyst nematode resistance gene Cre8,the eyespot resistance gene Pch1,the stem rust resistance gene Sr2 and Sr36,the leaf rust resistance gene Lr21,Lr34,Lr47 and Lr67,the tan spot resistance gene Tsn1 cannot be detected in all the tested materials.Most Yangmai cultivars(lines)were soft wheat,which was due to the Pinb-D1a genotype of 50%tested materials at Glu-A1 locus,2+12 at Glu-D1 of 86.7%tested materials and c at Glu-B3 locus of all tested materials except Yangmai3.In this study,the important functional genetic constitution of Yangmai series wheat cultivars(lines)was confirmed.The results can provide theoretical basis for production and breeding of Yangmai series wheat cultivars.