摘要
[Objectives]This study was conducted to establish a tissue culture regeneration system in Bama hemp(Cannabis sativa L.).[Methods]Using hemp seeds as explants,a regeneration system was established through explant sterilization,callus induction,callus differentiation,and rooting culture.[Results]The results showed that the best sterilization effect was achieved when sterilizing with 75%ethanol for 30 s,followed by 0.1%HgCl 2 solution for 9 min,with a contamination rate as low as 11.4%.In presence of 3 mg/L 2,4-D and 0.1 mg/L6-BA,the callus induction effect from hemp seeds was better.The formula for better differentiation of callus was MS+2.0 mg/L 6-BA+0.2 mg/L NAA.IBA had a promoting effect on the rooting of hemp aseptic plantlets.The highest rooting rate reached 80%when MS+0.3 mg/L IBA were used.[Conclusions]This study established a hemp seed regeneration system to provide technical support for the conservation and breeding of hemp germplasm resources.
[Objectives] This study was conducted to establish a tissue culture regeneration system in Bama hemp（Cannabis sativa L.）.[Methods] Using hemp seeds as explants, a regeneration system was established through explant sterilization, callus induction, callus differentiation, and rooting culture. [Results] The results showed that the best sterilization effect was achieved when sterilizing with 75% ethanol for 30 s, followed by 0.1% HgCl2 solution for 9 min, with a contamination rate as low as 11.4%. In presence of 3 mg/L 2,4-D and 0.1 mg/L6-BA, the callus induction effect from hemp seeds was better. The formula for better differentiation of callus was MS+2.0 mg/L 6-BA+0.2 mg/L NAA. IBA had a promoting effect on the rooting of hemp aseptic plantlets. The highest rooting rate reached 80% when MS+0.3 mg/L IBA were used. [Conclusions] This study established a hemp seed regeneration system to provide technical support for the conservation and breeding of hemp germplasm resources.
基金
Supported by Guangxi Natural Science Foundation(2017JJB130027)
The Basic Ability Enhancement Program for Young and Middle-aged Teachers of Guangxi(2017KY0724)
Master’s Degree Authorization Unit Construction Authorization Point(GXW[2018]7)
