长链非编码RNA MCM3AP-AS1在老年晚期乳腺癌患者肿瘤组织中的表达及临床病理意义

Expression and clinicopathological significance of long noncoding RNA MCM3AP-AS1 in tumor tissues of elderly patients with advanced breast cancer

目的:探讨长链非编码RNA(long noncoding RNA,LncRNA)MCM3AP-AS1在乳腺癌患者中的表达及其临床病理意义。方法:收集老年女性(年龄≥60岁)晚期乳腺癌患者肿瘤组织穿刺标本64例,应用qRT-PCR技术检测肿瘤组织中的LncRNA MCM3AP-AS1表达情况,免疫组化检测乳腺癌组织样本中的雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、人表皮生长因子受体-2(human epidermal growth factor receptor-2,Her-2)、Ki-67抗原、Dickkopf相关蛋白1(Dickkopf-related protein 1,Dkk1)蛋白表达情况。Her-2免疫组化结果2+的患者进一步行荧光原位杂交试验(FISH)检测。分析LncRNA MCM3AP-AS1表达情况与乳腺癌患者临床病理之间的关系及对预后的影响。结果:组织分化差及ER、PR阴性的乳腺癌患者肿瘤组织中LncRNA MCM3AP-AS1的表达明显升高(P <0.01)。LncRNA MCM3AP-AS1的表达与是否存在肺、肝、脑转移均无相关性,但与骨转移关系密切(P <0.01)。激素受体阴性、Her-2阳性、Dkk1阳性的患者更容易发生骨转移(P <0.01)。LncRNA MCM3AP-AS1高表达的乳腺癌患者无进展生存期明显低于低表达组(P <0.01)。结论:LncRNA MCM3APAS1与女性乳腺癌的恶性生物学行为关系密切。检测LncRNA MCM3AP-AS1表达对明确乳腺癌发病、转移机制及判断乳腺癌患者预后具有潜在的价值。

Objective:To investigate the expression and clinical pathological significance of long noncoding RNA(LncRNA) MCM3 AP-AS1 in breast cancer patients. Methods:A total of 64 cases of tumor tissue puncture specimens were collected from elderly women(age≥60)with advanced breast cancer. The expression of LncRNA MCM3 AP-AS1 in tumor tissues of these patients was detected by q RT-PCR. The expressions of ER,PR,Her-2,Ki-67 and Dkk1 protein in breast cancer tissue samples were detected by immunohistochemistry. Samples with immunohistochemical Her-2 2 + were further tested by FISH. Furthermore,we analyzed the relationship between LncRNA MCM3 AP-AS1 expression and clinical pathology of breast cancer patients and its impact on prognosis.Results:The expression of LncRNA MCM3 AP-AS1 was significantly increased in breast cancer patients with poor tissue differentiation and ER(-),PR(-)(P < 0.01). The expression of LncRNA MCM3 AP-AS1 was not associated with the presence of liver,lung or brain metastasis,but was closely related to bone metastasis(P < 0.01). Patients with ER(-),PR(-),Her-2(+)or Dkk1(+)were more likely to have bone metastases(P < 0.01). The progression-free survival of breast cancer patients with high expression of LncRNA MCM3 AP-AS1 was significantly shorter than that of low expression group(P < 0.01). Conclusion:LncRNA MCM3 AP-AS1 is closely related to the malignant biological behavior of female breast cancer. The detection of LncRNA MCM3 AP-AS1 expression has potential value in helping us to better understand the mechanism of breast cancer and judging the prognosis of breast cancer patients.